ArticleeLife2019
Programmed genome editing of the omega-1 ribonuclease of the blood fluke,
Open access · goldAbstract read
In one paragraph
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
13.5field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed, 106 citations in OpenAlex.
- Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing ofScience advances · 2026Article
- Heritable transgenic schistosomes as a living platform for SARS-CoV-2 neutralizing antibody secretion.Nature communications · 2026Article
- Gene Editing of a Carcinogenic Liver Fluke Tetraspanin Impairs Parasite Surface Biogenesis and Extracellular Vesicle Uptake by Human Host Cells.The Journal of infectious diseases · 2026Article
- TrueFrontiers in cellular and infection microbiology · 2026Article
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Current trends in application of CRISPR/Cas9 in gene editing and diagnostics in Neglected tropical diseases (NTDs).Molecular biology reports · 2025Review
- Clinical challenges and technological breakthroughs in helminthic therapy for diabetes.Frontiers in immunology · 2025Review
- Applications of CRISPR in Parasitology.Current pharmaceutical biotechnology · 2025Review
- CRISPR-based functional genomics for schistosomes and related flatworms.Trends in parasitology · 2024Review
- Review
- Transmission-Blocking Vaccines against Schistosomiasis Japonica.International journal of molecular sciences · 2024Review
- Schistosome Transgenesis: The Long Road to Success.Biology · 2024Review
- Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024Article
- Schistosoma antigens: A future clinical magic bullet for autoimmune diseases?Parasite (Paris, France) · 2024Review
- Schistosomiasis.Advances in experimental medicine and biology · 2024Review
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Review
- Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,Cell reports methods · 2023Article
- Gene editing in the nematode parasiteFrontiers in parasitology · 2023Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
21 authors at 9 institutions in 4 countries.
Wannaporn IttiprasertDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.ORCID 0000-0001-9411-8883
Victoria H MannDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.
Shannon E KarinshakResearch Center for Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.ORCID 0000-0002-2079-0973
Avril CoghlanWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Gabriel RinaldiWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Geetha SankaranarayananWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Apisit ChaideeResearch Center for Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.
Toshihiko TannoDepartment of Surgery, University of Maryland, Baltimore, United States.
Chutima KumkhaekCellular and Molecular Therapeutics Laboratory, National Heart, Lungs and Blood Institute, National Institutes of Health, Bethesda, United States.
Pannathee PrangtawornResearch Center for Neglected Diseases of Poverty, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.
Margaret M Mentink-KaneSchistosomiasis Resource Center, Biomedical Research Institute, Rockville, United States.
Christina J CochranDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.
Patrick DriguezWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Nancy HolroydWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Alan TraceyWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Rutchanee RodpaiDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Bart EvertsDepartment of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Cornelis H HokkeDepartment of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Karl F HoffmannInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United Kingdom.
Matthew BerrimanWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.ORCID 0000-0002-9581-0377
Paul J BrindleyDepartment of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington, DC, United States.ORCID 0000-0003-1765-0002
Wellcome Sanger Institute · GBGeorge Washington University · USKhon Kaen University · THLeiden University Medical Center · NLAberystwyth University · GBBiomedical Research Institute · USNational Institutes of Health · USSiriraj Hospital · THUniversity of Maryland, Baltimore · US
Funding
Antibiotic selection for schistosome transgenesisR21AI109532 · NIAID · GEORGE WASHINGTON UNIVERSITY · PI BRINDLEY, PAUL J, RINALDI, JOSE GABRIEL · 2014 to 2015
$436kNational Institute of Allergy and Infectious Diseases R21AI109532NIAID NIH HHS HHSN272201000005CNIAID NIH HHS HHSN272201000005INIAID NIH HHS R21 AI109532Royal Golden Jubilee Ph.D Program, Thailand PHD/0047/2556Thailand Research Fund PHD/0011/2555Thailand Research Fund PHD/0047/2556Thailand Research Fund PHD/00531/2556Wellcome 107475/Z/15/ZWellcome WT 098051Wellcome Trust
Abstract
CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of
Indexed as
Gene EditingAnimalsBase SequenceCell LineChromosomesCRISPR-Cas SystemsDNA RepairExonsGene Expression RegulationGenetic LociGranulomaHomologous RecombinationHumansInflammationLungMiceRibonucleasesRNA, MessengerCRISPR/Cas9granulomainfectious diseasemicrobiologyomega-1ribonucleaseSchistosoma mansoniTh2-polarization
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Read underepoch 390
Registered trials
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.