ArticleActa parasitologica2019
Molecular Phylodiagnosis of Enterocytozoon bieneusi and Encephalitozoon intestinalis in Children with Cancer: Microsporidia in Malignancies as an Emerging Opportunistic Infection.
Article in Acta parasitologica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Global prevalence and risk factors of Enterocytozoon bieneusi infection in humans: a systematic review and meta-analysis.Parasite (Paris, France) · 2024Pooled it
- Global epidemiology of Giardia duodenalis infection in cancer patients: a systematic review and meta-analysis.International health · 2022Pooled it
- Genotyping and molecular profiling of intestinal microsporidiosis and cryptosporidiosis in HIV-infected patients in Alborz Province, Iran.Gut pathogens · 2025Article
- Molecular evidence ofParasite epidemiology and control · 2025Article
- Identifying zoonotic risks: molecular insights into Cryptosporidium and Enterocytozoon bieneusi in pediatric cancer patients in Ahvaz, 2024.Parasitology research · 2025Article
- Microscopic and Molecular Investigation of Intestinal Microsporidia in HIV + /AIDS and Cancer Patients Undergoing Chemotherapy in Mazandaran Province, North of Iran.Acta parasitologica · 2023Article
- Molecular Prevalence of Microsporidia Infection in Patients with Lung Cancer.The American journal of tropical medicine and hygiene · 2023Article
- Occurrence of microsporidia spp. infection in children with diarrhea in southern Iran.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Article
- Chronic Infections in Mammals Due to Microsporidia.Experientia supplementum (2012) · 2022Article
- Prevalence and Genetic Characterization of Cryptosporidium and Microsporidia Infecting Hematological Malignancy Patients.Acta parasitologica · 2021Article
- A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae).Molecular ecology resources · 2020Article
- Zoonotic potential of Enterocytozoon bieneusi and Giardia duodenalis in horses and donkeys in northern China.Parasitology research · 2020Article
- Innate and Adaptive Immune Responses Against Microsporidia Infection in Mammals.Frontiers in microbiology · 2020Review
- Cyclophosphamide Treatment Mimics Sub-Lethal Infections WithFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrosporidia may cause infection in both immunocompromised and immunocompetent populations. The best strategy to control microsporidiosis is obtaining thorough knowledge of its outbreak and pathogenicity. PURPOSE : Because of the lack of precise estimation of microsporidia prevalence among Iranian children with cancer, the current study aimed at evaluating the rate of intestinal microsporidia in children undergoing chemotherapy.
methodsPatients with cancer undergoing chemotherapy in a children's hospital in Northwestern Iran were studied; 132 stool samples were collected and stained by the Weber and Ryan-blue modified trichrome staining techniques. The extracted DNA samples were evaluated by the nested polymerase chain reaction (PCR) method. All positive isolates were sequenced for genotyping and phylogenetic analysis.
resultsA total of 17 (12.8%) samples were microscopically positive for microsporidia infection, whereas only 14 (10.6%) cases were positive based on nested PCR results. In the positive samples detected with nested PCR, the frequency of Enterocytozoon bieneusi and Encephalitozoon intestinalis infections was 71.4% (n = 10) and 28.6% (n = 4), respectively. After sequencing and phylogenetic analysis, the genotype of E. bieneusi was type D and the sequences of the isolated species were similar to those of the registered ones.
conclusionE. bieneusi is a major contributor to microsporidiosis in young immunocompromised patients in Iran. Microsporidia species are well-detected when confirmatory techniques such as molecular methods are in agreement with staining. So, to ensure this, a suggestion has been made to introduce a certain diagnostic test for microsporidiosis.
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