ArticleNature chemistry2019
Supercharging enables organized assembly of synthetic biomolecules.
Article in Nature chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Effective charge saturation in ferritin cages.Scientific reports · 2026Article
- Inverse Design of DNA-Programmable Protein Colloidal Crystals.Journal of the American Chemical Society · 2025Article
- Engineering Protein-Polyelectrolyte Interactions for Cellular Applications.Annual review of chemical and biomolecular engineering · 2025Review
- Supercharged fluorescent proteins detect lanthanides via direct antennae signaling.Nature communications · 2024Article
- Protein Charge Neutralization Is the Proximate Driver Dynamically Tuning Reflectin Assembly.International journal of molecular sciences · 2024Article
- Convoluted micellar morphological transitions driven by tailorable mesogenic ordering effect from discotic mesogen-containing block copolymer.Nature communications · 2024Article
- Emerging Landscape of Supercharged Proteins and Peptides for Drug Delivery.ACS pharmacology & translational science · 2024Review
- Article
- Protein charge parameters that influence stability and cellular internalization of polyelectrolyte complex micelles.Chemical science · 2022Article
- Article
- Tailorable Tetrahelical Bundles as a Toolkit for Redox Studies.The journal of physical chemistry. B · 2022Article
- Understanding Supramolecular Assembly of Supercharged Proteins.ACS central science · 2022Article
- Article
- Charge Engineering Improves the Performance of Bst DNA Polymerase Fusions.ACS synthetic biology · 2022Article
- High affinity protein surface binding through co-engineering of nanoparticles and proteins.Nanoscale · 2022Article
- Enhanced immunogenicity of a positively supercharged archaeon thioredoxin scaffold as a cell-penetrating antigen carrier for peptide vaccines.Frontiers in immunology · 2022Article
- Knowledge-Based Unfolded State Model for Protein Design.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Protein Assembly by Design.Chemical reviews · 2021Review
- Decorated networks of native proteins: nanomaterials with tunable mesoscopic domain size.Soft matter · 2021Article
- Redefining Protein Interfaces within Protein Single Crystals with DNA.Journal of the American Chemical Society · 2021Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However, there are few methods for constructing such oligomers. Here we have engineered completely synthetic, symmetrical oligomers by combining pairs of oppositely supercharged variants of a normally monomeric model protein through a strategy we term 'supercharged protein assembly' (SuPrA). We show that supercharged variants of green fluorescent protein can assemble into a variety of architectures including a well-defined symmetrical 16-mer structure that we solved using cryo-electron microscopy at 3.47 Å resolution. The 16-mer is composed of two stacked rings of octamers, in which the octamers contain supercharged proteins of alternating charges, and interactions within and between the rings are mediated by a variety of specific electrostatic contacts. The ready assembly of this structure suggests that combining oppositely supercharged pairs of protein variants may provide broad opportunities for generating novel architectures via otherwise unprogrammed interactions.
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Registered trials
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