ArticleScientific reports2019
Manipulation of the precursor supply for high-level production of longifolene by metabolically engineered Escherichia coli.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Combining multiple metabolic strategies for efficient production of longifolene inSynthetic and systems biotechnology · 2026Article
- Chemoenzymatic Synthesis of Structurally Diverse Terpenoids from Farnesyl Pyrophosphates Modified at the Central Alkene Unit.Journal of the American Chemical Society · 2026Article
- Article
- Novel phenoxyacetic herbicides synthesized from longifolene-derived primary amine for sustainable weed management.RSC advances · 2025Article
- Optimizing longifolene production inSynthetic and systems biotechnology · 2025Article
- Engineering a Biosynthetic Pathway to Produce (+)-Brevianamides A and B.ACS catalysis · 2025Article
- Towards sustainable agarwood production: integrating microbial interactions, anatomical changes, and metabolite biosynthesis.Journal of industrial microbiology & biotechnology · 2024Review
- Review
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- The role of volatile organic compounds for assessing characteristics and severity of non-cystic fibrosis bronchiectasis: an observational study.Frontiers in medicine · 2024Article
- Article
- Highly efficient biosynthesis of β-caryophyllene with a new sesquiterpene synthase from tobacco.Biotechnology for biofuels and bioproducts · 2022Article
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Synthesis of Ferulenol by EngineeredMolecules (Basel, Switzerland) · 2021Article
- Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol.BMC biotechnology · 2021Article
- Whole-Cell Production of Patchouli Oil Sesquiterpenes inACS omega · 2020Article
- Metabolic engineering ofJournal of biological research (Thessalonike, Greece) · 2020Article
- Production of Terpenoids by Synthetic Biology Approaches.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- Electricigens in the anode of microbial fuel cells: pure cultures versus mixed communities.Microbial cell factories · 2019Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Longifolene is a naturally occurring tricyclic sesquiterpene widely used in many different fields. Up to now, this valuable terpene was mainly manufactured from the high-boiling fraction of certain pine resins. Microbial production can be a promising alternative to the extraction from natural plant sources. Here, we present the metabolic engineering strategy to assemble biosynthetic pathway for longifolene production in Escherichia coli. E. coli was rendered to produce longifolene by heterologously expressing a codon optimized longifolene synthase from Picea abies. Augmentation of the metabolic flux to farnesyl pyrophosphate (FPP) by different FPP synthases conferred a 1.8-fold increase in longifolene production. An additional enhancement of longifolene production (up to 2.64 mg/L) was achieved by introducing an exogenous mevalonate pathway. Under fed-batch conditions, the best-performing strain was able to produce 382 mg/L of longifolene in a 5 L bioreactor. These results demonstrated the feasibility of producing longifolene by microbial fermentation and could serve as the basis for the construction of more robust strains in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.