ArticlePloS one2019
Metagenomic analysis of viruses in toilet waste from long distance flights-A new procedure for global infectious disease surveillance.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes.Microbiology spectrum · 2022Pooled it
- International Airport Wastewater as a Sentinel Site for Genomic Surveillance of Human Viruses and Bacteriophages.Microorganisms · 2026Article
- Reactivation and Management of Endogenous Latent Herpesviruses in the Spaceflight Environment.Current microbiology · 2026Review
- Effect of nucleic acid concentration method on Severe Acute Respiratory Syndrome Coronavirus 2 detection sensitivity in aircraft wastewater.Journal of medical microbiology · 2026Article
- Aircraft lavatory wastewater surveillance for movement of antimicrobial resistance genes: a proof-of-concept study.Microbiology spectrum · 2025Article
- Metagenomic analysis of human, animal, and environmental samples identifies potential emerging pathogens, profiles antibiotic resistance genes, and reveals horizontal gene transfer dynamics.Scientific reports · 2025Article
- Comparison of Three Viral Nucleic Acid Preamplification Pipelines for Sewage Viral Metagenomics.Food and environmental virology · 2024Article
- Expert consensus on One Health for establishing an enhanced and integrated surveillance system for key infectious diseases.Infectious medicine · 2024Article
- Metagenomic analysis of sewage for surveillance of bacterial pathogens: A release experiment to determine sensitivity.PloS one · 2024Article
- Quantitatively assessing early detection strategies for mitigating COVID-19 and future pandemics.Nature communications · 2023Article
- Quantitatively assessing early detection strategies for mitigating COVID-19 and future pandemics.medRxiv : the preprint server for health sciences · 2023Article
- Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters.Applied and environmental microbiology · 2023Article
- Suitability of aircraft wastewater for pathogen detection and public health surveillance.The Science of the total environment · 2023Article
- Evaluation of deep space exploration risks and mitigations against radiation and microgravity.Frontiers in nuclear medicine · 2023Review
- Viral metagenomics reveals persistent as well as dietary acquired viruses in Antarctic fur seals.Scientific reports · 2022Article
- Article
- Spaceflight Virology: What Do We Know about Viral Threats in the Spaceflight Environment?Astrobiology · 2022Review
- Exploring the diversity of coronavirus in sewage during COVID-19 pandemic: Don't miss the forest for the trees.The Science of the total environment · 2021Article
- NGS Techniques Reveal a High Diversity of RNA Viral Pathogens and Papillomaviruses in Fresh Produce and Irrigation Water.Foods (Basel, Switzerland) · 2021Article
- Porcine reproductive and respiratory syndrome virus whole-genome sequencing efficacy with field clinical samples using a poly(A)-tail viral genome purification method.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human viral pathogens are a major public health threat. Reliable information that accurately describes and characterizes the global occurrence and transmission of human viruses is essential to support national and global priority setting, public health actions, and treatment decisions. However, large areas of the globe are currently without surveillance due to limited health care infrastructure and lack of international cooperation. We propose a novel surveillance strategy, using metagenomic analysis of toilet material from international air flights as a method for worldwide viral disease surveillance. The aim of this study was to design, implement, and evaluate a method for viral analysis of airplane toilet waste enabling simultaneous detection and quantification of a wide range of human viral pathogens. Toilet waste from 19 international airplanes was analyzed for viral content, using viral capture probes followed by high-throughput sequencing. Numerous human pathogens were detected including enteric and respiratory viruses. Several geographic trends were observed with samples originating from South Asia having significantly higher viral species richness as well as higher abundances of salivirus A, aichivirus A and enterovirus B, compared to samples originating from North Asia and North America. In addition, certain city specific trends were observed, including high numbers of rotaviruses in airplanes departing from Islamabad. Based on this study we believe that central sampling and analysis at international airports could be a useful supplement for global viral surveillance, valuable for outbreak detection and for guiding public health resources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.