ArticleTheriogenology2019
Bovine eggs release zinc in response to parthenogenetic and sperm-induced egg activation.
Article in Theriogenology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Extracellular zinc acts as a dynamic extracellular regulator of fertilization in Xenopus laevis†.Biology of reproduction · 2026Article
- A comprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals.The Canadian journal of urology · 2026Review
- What we could not have known: ovarian cycles, follicle development, and the signature of life.Molecular human reproduction · 2026Review
- The oocyte zinc transportereLife · 2025Article
- Article
- Zinc transporter ZnT3/Slc30a3 has a potential role in zinc ion influx in mouse oocytes.The Journal of reproduction and development · 2024Article
- Molecular Mechanism of Oocyte Activation in Mammals: Past, Present, and Future Directions.Biomolecules · 2024Review
- ZneLife · 2023Article
- Phospholipase C Zeta 1 (PLCZ1): The Function and Potential for Fertility Assessment and In Vitro Embryo Production in Cattle and Horses.Veterinary sciences · 2023Review
- ZnbioRxiv : the preprint server for biology · 2023Article
- The Therapeutic and Diagnostic Potential of Phospholipase C Zeta, Oocyte Activation, and Calcium in Treating Human Infertility.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation.Animals : an open access journal from MDPI · 2022Article
- Quantitative imaging approaches to understanding biological processing of metal ions.Current opinion in chemical biology · 2022Review
- Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington's Disease.International journal of molecular sciences · 2022Review
- Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application.Human reproduction open · 2022Review
- Review
- Metal ion fluxes controlling amphibian fertilization.Nature chemistry · 2021Article
- Role of zinc in female reproduction.Biology of reproduction · 2021Review
- The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish.Scientific reports · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Upon fertilization or parthenogenesis, zinc is released into the extracellular space through a series of exocytic events termed zinc sparks, which are tightly coordinated with intracellular calcium transients. The zinc spark reduces the total amount of intracellular zinc, and this reduction is necessary and sufficient to induce egg activation even in the absence of calcium transients. In addition, this zinc release contributes to the block to polyspermy through modification of the zona pellucida. The zinc spark has been documented in all organisms examined to date including the mouse, two species of nonhuman primates, and human. Here we determined whether zinc sparks occur in the bovine, an important model of gamete development in mono-ovulatory mammalian species. We obtained metaphase II-arrested (MII) bovine eggs following in vitro maturation. Total zinc, assessed in single cells using X-Ray Fluorescence Microscopy, was significantly more abundant in the bovine egg compared to iron and copper. Studies with intracellular fluorescent probes revealed that labile zinc pools are localized to discrete cytoplasmic punctae enriched at the cortex. To determine whether zinc undergoes dynamic fluxes during egg activation, we parthenogenetically activated bovine eggs using two approaches: ionomycin or bovine phospholipase C zeta (bPlcζ). Both these methods induced zinc sparks coordinately with intracellular calcium transients. The zinc spark was also observed in bovine eggs following intracytoplasmic sperm injection. These results establish that zinc is the most abundant transition metal in the bovine egg, and zinc flux during egg activation - induced by chemical activation or sperm - is a highly conserved event across mammalian species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.