ArticleMikrochimica acta2019
Fluorometric determination of the activity of uracil-DNA glycosylase by using graphene oxide and exonuclease I assisted signal amplification.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 25 citations in OpenAlex.
- A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles.Biosensors · 2023Article
- Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity.Open biology · 2021Article
- Amplified Fluorescent Aptasensor for Ochratoxin A Assay Based on Graphene Oxide and RecJToxins · 2020Article
- A robust photoluminescence screening assay identifies uracil-DNA glycosylase inhibitors against prostate cancer.Chemical science · 2020Article
- Recent advances in graphene-based nanomaterials: properties, toxicity and applications in chemistry, biology and medicine.Mikrochimica acta · 2019Review
- Fluorescent Method for the Detection of Biothiols Using an Ag⁺-Mediated Conformational Switch.Sensors (Basel, Switzerland) · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The base-excision repair enzyme uracil-DNA glycosylase (UDG) plays a crucial role in the maintenance of genome integrity. The authors describe a fluorometric method for the detection of the activity of UDG. It is making use of (a) a 3'-FAM-labeled hairpin DNA probe with two uracil deoxyribonucleotides in the self-complementary duplex region of its hairpin structure, (b) exonuclease I (Exo I) that catalyzes the release of FAM from the UDG-induced stretched ssDNA probe, and (c) graphene oxide that quenches the green FAM fluorescence of the intact hairpin DNA probe in the absence of UDG. If Exo I causes the release of FAM from the hairpin DNA probe, the fluorescence peaking at 517 nm is turned off in the absence of UDG but turned on in its presence. The resulting assay has a wide linear range (0.008 to 1 U·mL
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