ArticleMikrochimica acta2019
Colorimetric detection and typing of E. coli lipopolysaccharides based on a dual aptamer-functionalized gold nanoparticle probe.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 58 citations in OpenAlex.
- Reduced graphene oxide-based absorbance biosensors for detecting Escherichia coli DNA.Scientific reports · 2025Article
- Recent nanoengineered therapeutic advancements in sepsis management.Frontiers in bioengineering and biotechnology · 2024Review
- Metallic and Metal Oxides Nanoparticles for Sensing Food Pathogens-An Overview of Recent Findings and Future Prospects.Materials (Basel, Switzerland) · 2022Review
- Recent advances in aptamer applications for analytical biochemistry.Analytical biochemistry · 2022Article
- Review
- Recent progress in the optical detection of pathogenic bacteria based on noble metal nanoparticles.Mikrochimica acta · 2021Review
- Review
- Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications.International journal of molecular sciences · 2020Review
- An electrochemical sensor for bacterial lipopolysaccharide detection based on dual functional CuMikrochimica acta · 2020Article
- G-quadruplex-based assay combined with aptamer and gold nanoparticles for Escherichia coli K88 determination.Mikrochimica acta · 2020Article
- Detection ofSensors (Basel, Switzerland) · 2020Article
- A fluorescence quenching based gene assay for Escherichia coli O157:H7 using graphene quantum dots and gold nanoparticles.Mikrochimica acta · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A rapid method for identification and typing of lipopolysaccharides (LPS) was developed by utilizing the different binding affinities between two kinds of gold nanoparticles (AuNPs) functionalized with two aptamers. Aptamers against ethanolamine and E. coli O111:B4 LPS were used to functionalize the AuNPs. The AuNPs functionalized with ethanolamine aptamer can bind to ethanolamine and are termed general probe (G-probe). The G-probe can recognize any type of LPS because ethanolamine is a component of every type of LPS. This causes a sandwich-mediated aggregation of the AuNPs and a color change from red to blue. The AuNPs functionalized with aptamer against the LPS of E. coli O111:B4 specifically bind to O111:B4 LPS and are termed specific probe (S-probe). By using these two probes, a logic typing method was developed. It can detect LPS in concentrations between 2.5 and 20 μg·mL
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Registered trials
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