ArticleJournal of assisted reproduction and genetics2019
Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Fertilization time and oocyte spindle integrity: implication for embryo ploidy.Reproduction & fertility · 2026Article
- MOL classification: a novel meiotic spindle scoring system for predicting human embryo development and ploidy.Journal of assisted reproduction and genetics · 2026Article
- Spindle-to-oocyte light retardance ratio as a noninvasive biomarker for oocyte quality assessment: a prospective cohort study.Frontiers in endocrinology · 2026Article
- Intracytoplasmic Sperm Injection (ICSI) Timing Affects the Influence of Sperm DNA Fragmentation on ICSI Outcomes.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- The Istanbul consensus update: a revised ESHRE/ALPHA consensus on oocyte and embryo static and dynamic morphological assessment†,‡.Human reproduction (Oxford, England) · 2025Article
- Spindle observation aids decision-making for early rescue ICSI.Journal of assisted reproduction and genetics · 2025Article
- In Vitro Maturation and Time-Lapse Imaging of Immature Human Oocytes: A Direction Toward Clinical Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Histories and mysteries underlying meiotic maturation failure in human oocytes.Journal of assisted reproduction and genetics · 2024Article
- Cumulus cell DNA damage linked to fertilization success in females with an ovulatory dysfunction phenotype.Frontiers in cell and developmental biology · 2024Article
- RescueFrontiers in endocrinology · 2022Article
- Developmental potential of immature human oocytes aspirated after controlled ovarian stimulation.Journal of assisted reproduction and genetics · 2021Article
- Cytoplasmic maturation in human oocytes: an ultrastructural study †.Biology of reproduction · 2021Article
- Thoughts on the popularity of ICSI.Journal of assisted reproduction and genetics · 2021Review
- Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence.Animals : an open access journal from MDPI · 2020Review
- ICSI in late matured oocytes, is it worth it? Study with laboratory, clinical and genetic evaluation results.JBRA assisted reproduction · 2020Article
- A Study to Find Optimal Intra-cytoplasmic Sperm Injection Timing of Oocytes Matured from Germinal Vesicle inJournal of human reproductive sciencesArticle
- Revisiting the Trigger-to-Oocyte Retrieval Interval: A Machine Learning-Guided Strategy for Improving Oocyte Yield in Diminished Ovarian Reserve.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
proposeThe presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI).
methodsThe study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern.
resultsThe visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded.
conclusionsThe study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage.
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