ArticleThe Journal of biological chemistry2019
Skeletal muscle-specific knockout of DEP domain containing 5 protein increases mTORC1 signaling, muscle cell hypertrophy, and mitochondrial respiration.
Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- High-intensity interval training in aged female mice preserves physical, cognitive, and cardiovascular function.GeroScience · 2026Article
- Amino Acid Metabolism in Health and Disease.MedComm · 2026Review
- Constitutive mTORC1 activation in skeletal muscle increases inflammation but is not sufficient to impair glucose tolerance.Function (Oxford, England) · 2026Article
- Hyperactive muscle mTORC1 attenuates functional adaptations to endurance training despite alterations in mitochondrial and lipid profiles.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- From Deficiency to Therapy: Systemic Consequences of ALAS1 Disruption and the Protective Role of 5-ALA.Life (Basel, Switzerland) · 2025Review
- Constitutively active mTORC1 signaling modifies the skeletal muscle metabolome and lipidome response to exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2025Article
- Endurance exercise preserves physical function in adult and older male C57BL/6 mice: high intensity interval training (HIIT)Frontiers in aging · 2024Article
- Metabolomic and Lipidomic Signature of Skeletal Muscle with Constitutively Active Mechanistic Target of Rapamycin Complex 1.The Journal of nutrition · 2023Article
- Review
- Skeletal Muscle Transcriptome Alterations Related to Declining Physical Function in Older Mice.Journal of ageing and longevity · 2023Article
- Dual roles of mTORC1-dependent activation of the ubiquitin-proteasome system in muscle proteostasis.Communications biology · 2022Article
- Article
- Biology of Activating Transcription Factor 4 (ATF4) and Its Role in Skeletal Muscle Atrophy.The Journal of nutrition · 2022Review
- Cardiac Investigations in Sudden Unexpected Death in DEPDC5-Related Epilepsy.Annals of neurology · 2022Article
- SEA and GATOR 10 Years Later.Cells · 2021Review
- Measuring Exercise Capacity and Physical Function in Adult and Older Mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- Cafeteria Diet Impacts the Body Weight and Energy Expenditure of Brown Norway Rats in an Apparent Age Dependent Manner, but Has no Effect on Muscle Anabolic Sensitivity to Nutrition.Frontiers in nutrition · 2021Article
- LINCing Nuclear Mechanobiology With Skeletal Muscle Mass and Function.Frontiers in cell and developmental biology · 2021Review
- Advances in the Role of Leucine-Sensing in the Regulation of Protein Synthesis in Aging Skeletal Muscle.Frontiers in cell and developmental biology · 2021Review
- ATF4 Is a Fundamental Regulator of Nutrient Sensing and Protein Turnover.The Journal of nutrition · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 6 countries.
Funding
Abstract
mTORC1 regulates protein synthesis and in turn is regulated by growth factors, energy status, and amino acid availability. In kidney cell (HEK293-T) culture, the GAP activity toward RAG (GATOR1) protein complex suppresses activation of the RAG A/B-RAG C/D heterodimer when amino acids are insufficient. During amino acid sufficiency, the RAG heterodimer recruits mTORC1 to the lysosomal membrane where its interaction with Ras homolog enriched in brain (Rheb) stimulates mTORC1's kinase activity. The DEP domain containing 5 (DEPDC5) protein, a GATOR1 subunit, causes familial focal epilepsy when mutated, and global knockout of the Depdc5 gene is embryonically lethal. To study the function of DEPDC5 in skeletal muscle, we generated a muscle-specific inducible Depdc5 knockout mouse, hypothesizing that knocking out Depdc5 in muscle would make mTORC1 constitutively active, causing hypertrophy and improving muscle function. Examining mTORC1 signaling, morphology, mitochondrial respiratory capacity, contractile function, and applied physical function (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.