ArticleiScience2019
Derivation of Haploid Trophoblast Stem Cells via Conversion In Vitro.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Evolutionary Analysis of Transcriptional Regulation Mediated by Cdx2 in Rodents.Cell proliferation · 2026Article
- Development and application of haploid embryonic stem cells.Stem cell research & therapy · 2024Review
- Haploid-genetic screening of trophectoderm specification identifiesScience advances · 2023Article
- BCL2 is a major regulator of haploidy maintenance in murine embryonic stem cells.Cell proliferation · 2023Article
- Transcriptome profiles and chromatin states in mouse androgenetic haploid embryonic stem cells.Cell proliferation · 2023Article
- The immune checkpoint molecule, VTCN1/B7-H4, guides differentiation and suppresses proinflammatory responses and MHC class I expression in an embryonic stem cell-derived model of human trophoblast.Frontiers in endocrinology · 2023Article
- Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells.Cell proliferation · 2022Article
- Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells.STAR protocols · 2022Article
- Derivation of human triploid trophoblast stem cells.Journal of assisted reproduction and genetics · 2022Article
- Genome-wide screening in the haploid system reveals Slc25a43 as a target gene of oxidative toxicity.Cell death & disease · 2022Article
- Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs.Frontiers in cell and developmental biology · 2022Article
- Rapid generation of murine haploid-induced trophoblast stem cellsSTAR protocols · 2021Article
- High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.Stem cells translational medicine · 2021Article
- MicroRNAs as crucial mediators in the pharmacological activities of triptolide (Review).Experimental and therapeutic medicine · 2021Review
- Applications ofStem cells international · 2021Review
- Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence.Oncology reports · 2020Article
- Inhibition of Apoptosis Reduces Diploidization of Haploid Mouse Embryonic Stem Cells during Differentiation.Stem cell reports · 2020Article
- Establishment of an eHAP1 human haploid cell line hybrid reference genome assembled from short and long reads.Genomics · 2020Article
- Bioengineered microenvironment to culture early embryos.Cell proliferation · 2020Review
- The milestone of genetic screening: Mammalian haploid cells.Computational and structural biotechnology journal · 2020Review
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Owing to their single genome, haploid cells are powerful to uncover unknown genes by performing genetic screening in mammals. However, no haploid cell line from an extraembryonic lineage has been achieved yet, which limits the application of haploid cells in placental genetic screening. Here, we show that overexpression of Cdx2 can convert haploid embryonic stem cells to trophoblast stem cells (TSCs). p53 deletion reduces diploidization during the conversion and guarantees the generation of haploid-induced TSCs (haiTSCs). haiTSCs not only share the same molecular characterization with trophoderm-derived TSCs but also possess multipotency to placental lineages in various procedures. In addition, haiTSCs can maintain haploidy in the long term, assisted by periodic sorting and with reliance on FGF4 and heparin. Finally, we perform piggyBac-mediated high-throughput mutation in haiTSCs and use them in trophoblast lineage genetic screening. Deep sequencing analysis and validation experiments prove that Htra1 is a blocker for spongiotrophoblast specification.
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