ArticleBMC plant biology2019
Selection of reference genes for quantitative real-time PCR normalization in the plant pathogen Puccinia helianthi Schw.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 36 citations in OpenAlex.
- Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR.BMC plant biology · 2025Article
- Selection of reference genes in Thermothelomyces fergusii: insights from genomic and expression analysis in response to different carbon sources.BMC genomics · 2025Article
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- Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies inFrontiers in plant science · 2023Article
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- Reference Genes Selection ofJournal of fungi (Basel, Switzerland) · 2022Article
- Selection of potential reference genes for RT-qPCR in the plant pathogenic fungusFrontiers in microbiology · 2022Article
- Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages inOpen life sciences · 2022Article
- Reference genes identification for qRT-PCR normalization of gene expression analysis inFrontiers in plant science · 2022Article
- Identification of reference genes and their validation for gene expression analysis in phytopathogenic fungus Macrophomina phaseolina.PloS one · 2022Article
- Identification and Validation of Reference Genes for Gene Expression Analysis in Different Development Stages ofFrontiers in microbiology · 2021Article
- Selection of suitable reference genes for quantitative real time PCR in different Tulasnella isolates and orchid-fungus symbiotic germination system.Molecular biology reports · 2021Article
- Identification and Validation of Reference Genes inFrontiers in microbiology · 2021Article
- Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis.BMC plant biology · 2020Article
- Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis inBioMed research international · 2020Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundReal-time RT-PCR has become a common and robust technique to detect and quantify low-abundance mRNA expression and is a prefered tool when examining fungal gene expression in infected host tissues. However, correct evaluation of gene expression data requires accurate and reliable normalization against a reference transcript. Thus, the identification of reference genes with stable expression during different conditions is of paramount importance. Here, we present a study where in vitro and in planta experiments were used to validate the expression stability of reference gene candidates of Puccinia helianthi Schw., an obligate pathogen that causes rust in sunflower (Helianthus annuus).
resultsEleven reference genes of P. helianthi were validated at different growth stages. Excel-based software geNorm, BestKeeper and NormFinder were used to evaluate the reference gene transcript stabilities. Of eleven reference gene candidates tested, three were stably expressed in urediniospores, germinating growth stage and in planta. Two of these genes (UBC, EF2), encoding ubiquitin-conjugating enzyme and elongation factor 2, proved to be the most stable set of reference genes under the experimental conditions used.
conclusionWe found that UBC and EF2 are suitable candidates for for the standardization of gene expression studies in the plant pathogen P. helianthi and potentially other related pathogens.
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