ArticleiScience2019
A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- Transgenerational inheritance of altered H3K27me3 in wild-type Caenorhabditis elegans.The EMBO journal · 2026Article
- DivergenteLife · 2026Article
- Suppression of Tau Phosphorylation Induces Neurotoxicity, Causing Developmental Defects and Degeneration inCells · 2026Article
- HSP-1-specific nanobodies alter chaperone function in vitro and in vivo.The Journal of biological chemistry · 2026Article
- TAG-IN, a swappable strategy for endogenous gene tagging inbioRxiv : the preprint server for biology · 2026Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.PLoS genetics · 2025Article
- Article
- A cGAL-UAS bipartite expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A toolkit for assembly of targeting clones formicroPublication biology · 2023Article
- CRISPR-activated expression of collagenmicroPublication biology · 2023Article
- The Q-system: A Versatile Repressible Binary Expression System.Methods in molecular biology (Clifton, N.J.) · 2022Review
- IQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish.Communications biology · 2021Article
- Improved GAL4 and Tet OFF drivers formicroPublication biology · 2021Article
- A novel in vitro Caenorhabditis elegans transcription system.BMC molecular and cell biology · 2020Article
- Efficient Transgenesis inGenetics · 2020Article
- TheGenetics · 2019Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Binary gene regulatory tools such as the Tetracycline (Tet)-controlled transcription system have revolutionized genetic research in multiple organisms, but their applications to the worm remain very limited. Here we report that the canonical Tet system is largely inactive in the worm but can be adapted for the worm by introducing multiple modifications, a crucial one being the use of the transcription activation domain from the fungal Q binary system. The resultant Tet/Q hybrid system proves more robust and flexible than either of its precursors, enabling elaborate modes of transgene manipulation previously hard to achieve in the worm, including inducible intersectional regulation and, in combination with the Q system, independent control of distinct transgenes within the same cells. Furthermore, we demonstrated, as an example of its applications, that the hybrid system can tightly and efficiently control Cre expression. This study establishes Tet/Q as a premier binary system for worm genetic research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.