Evidence map›Paper›PMID 30633912›Full record

ArticleCell2019

Circulating Tumor Cell Clustering Shapes DNA Methylation to Enable Metastasis Seeding.

Sofia Gkountela, Francesc Castro-Giner, Barbara Maria Szczerba, Marcus Vetter, Julia Landin, Ramona Scherrer, Ilona Krol, Manuel C Scheidmann, Christian Beisel, Christian U Stirnimann and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 509 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
509citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

509 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. [Liquid Biopsy Revolutionizes the Precise Management of Tumors Across the Entire Course: Current Situation and Future Prospects].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2026
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  10. Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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449 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Sofia GkountelaCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
Francesc Castro-GinerCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland; SIB Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland.
Barbara Maria SzczerbaCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
Marcus VetterGynecologic Cancer Center, University Hospital Basel, 4056 Basel, Switzerland; Department of Medical Oncology, University Hospital Basel, 4056 Basel, Switzerland.
Julia LandinDepartment of Medical Oncology, University Hospital Basel, 4056 Basel, Switzerland; Breast Center, University Hospital Basel and University of Basel, 4031 Basel, Switzerland.
Ramona ScherrerCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
Ilona KrolCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
Manuel C ScheidmannCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
Christian BeiselDepartment of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland.
Christian U StirnimannNEXUS Personalized Health Technologies, ETH Zurich, 8092 Zurich, Switzerland.
Christian KurzederGynecologic Cancer Center, University Hospital Basel, 4056 Basel, Switzerland; Breast Center, University Hospital Basel and University of Basel, 4031 Basel, Switzerland.
Viola Heinzelmann-SchwarzGynecologic Cancer Center, University Hospital Basel, 4056 Basel, Switzerland.
Christoph RochlitzDepartment of Medical Oncology, University Hospital Basel, 4056 Basel, Switzerland.
Walter Paul WeberBreast Center, University Hospital Basel and University of Basel, 4031 Basel, Switzerland.
Nicola AcetoCancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 4058 Basel, Switzerland. Electronic address: nicola.aceto@unibas.ch.

Funding

European Research Council 678834
6 · The paper itself

Abstract

The ability of circulating tumor cells (CTCs) to form clusters has been linked to increased metastatic potential. Yet biological features and vulnerabilities of CTC clusters remain largely unknown. Here, we profile the DNA methylation landscape of single CTCs and CTC clusters from breast cancer patients and mouse models on a genome-wide scale. We find that binding sites for stemness- and proliferation-associated transcription factors are specifically hypomethylated in CTC clusters, including binding sites for OCT4, NANOG, SOX2, and SIN3A, paralleling embryonic stem cell biology. Among 2,486 FDA-approved compounds, we identify Na

Indexed as

AnimalsBreast NeoplasmsCell DifferentiationCell Line, TumorCell ProliferationDisease Models, AnimalDNA MethylationFemaleHumansMiceMice, Inbred NODNanog Homeobox ProteinNeoplasm MetastasisNeoplastic Cells, CirculatingOctamer Transcription Factor-3Repressor ProteinsNanog Homeobox ProteinNANOG protein, humanOctamer Transcription Factor-3POU5F1 protein, humanRepressor ProteinsSIN3A transcription factorSin3 Histone Deacetylase and Corepressor ComplexSOX2 protein, humanSOXB1 Transcription Factorsbisulfite sequencingcirculating tumor cell clusterscirculating tumor cellsdrug screenproliferation-associated transcription factorsRNA sequencingsingle cell sequencingstemness-associated transcription factors

Identifiers

PMID30633912
PMCPMC6363966

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.