ArticleMikrochimica acta2019
Electrochemical determination of the activity and inhibition of telomerase based on the interaction of DNA with molybdate.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Development of a Novel Phagomagnetic-Assisted Isothermal DNA Amplification System for Endpoint Electrochemical Detection ofBiosensors · 2023Article
- Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability.Biosensors · 2022Review
- Electrochemical assay for analysis of circulation tumor cells based on isolation of the cell with magnetic nanoparticles and reaction of DNA with molybdate.Mikrochimica acta · 2020Article
- Gold nanoparticle-modified black phosphorus nanosheets with improved stability for detection of circulating tumor cells.Mikrochimica acta · 2020Article
- Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level.Sensors (Basel, Switzerland) · 2020Review
- Analysis of glycan expression on cell surfaces by using a glassy carbon electrode modified with MnOMikrochimica acta · 2020Article
- Target induced framework nucleic acid nanomachine with doxorubicin-spherical nucleic acid tags for electrochemical determination of human telomerase activity.Mikrochimica acta · 2020Article
- Amperometric genosensor for culture independent bacterial count.Sensors and actuators. B, Chemical · 2019Article
- Molecularly imprinted gelatin nanoparticles for DNA delivery and in-situ fluorescence imaging of telomerase activity.Mikrochimica acta · 2019Article
- Electrochemical sensing of L-ascorbic acid by using a glassy carbon electrode modified with a molybdophosphate film.Mikrochimica acta · 2019Article
- Electrochemical immunoassay for the tumor marker CD25 by coupling magnetic sphere-based enrichment and DNA based signal amplification.Mikrochimica acta · 2019Article
- Aptamer based determination of the cancer biomarker HER2 by using phosphate-functionalized MnOMikrochimica acta · 2019Article
- Cobalt phosphide nanowires for fluorometric detection and in-situ imaging of telomerase activity via hybridization chain reactions.Mikrochimica acta · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
An ultrasensitive electrochemical sensor is described for the determination of the activity of telomerase. It is based on a DNA-generated current that is due to the reaction of the phosphate groups on DNA with molybdate to form a redox-active molybdophosphate. A telomerase substrate primer was first immobilized on a gold electrode. In the presence of telomerase and deoxyribonucleoside triphosphates (dNTPs), the primer can be extended with repetitive nucleotide sequences (TTAGGG). The subsequent reaction of the sensor with molybdate results in the enhancement of electrochemical current intensity due to an increased amount of nucleotides on the electrode. Sensitivity can be further improved by introducing a hairpin probe that partially hybridizes with the repetitive TTAGGG sequence and further enhances the amount of DNA on the electrode. The biosensor, best operated at 0.2 V (vs. Ag/AgCl) shows a linear response to telomerase activity from 1×10
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