Evidence map›Paper›PMID 30631309›Full record

ArticleFrontiers in microbiology2018

Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria.

Rudolf O Schlechter, Hyunwoo Jun, Michał Bernach, Simisola Oso, Erica Boyd, Dian A Muñoz-Lintz, Renwick C J Dobson, Daniela M Remus, Mitja N P Remus-Emsermann

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 135 citations in OpenAlex.

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  8. Jumbo phage-mediated transduction of genomic islands.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  12. Frequency-dependent fitness effects are ubiquitous.bioRxiv : the preprint server for biology · 2025
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Rudolf O SchlechterSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Hyunwoo JunSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Michał BernachSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Simisola OsoSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Erica BoydSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Dian A Muñoz-LintzSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Renwick C J DobsonSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Daniela M RemusSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Mitja N P Remus-EmsermannSchool of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
University of Canterbury · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn

Indexed as

fluorescent labelingfluorophoretaggingTn5Tn7transposon

Identifiers

PMID30631309
PMCPMC6315172
OpenAlexW2951372251

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.