ArticleScientific reports2019
A new recombineering system for precise genome-editing in Shewanella oneidensis strain MR-1 using single-stranded oligonucleotides.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Deciphering the Mto electron uptake pathway ofApplied and environmental microbiology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Functional characterization of a type I-F1 CRISPR-cas system from the clinical isolate Shewanella xiamenensis Sh95 reveals constitutive activity and plasmid-curing capability.Scientific reports · 2026Article
- Type I-F CRISPR-associated transposons contribute to genomic plasticity inMicrobial genomics · 2025Article
- Article
- Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering.Engineering microbiology · 2024Review
- Multiple Rounds ofACS synthetic biology · 2023Article
- Characterization of the inner membrane cytochrome ImcH from Geobacter reveals its importance for extracellular electron transfer and energy conservation.Protein science : a publication of the Protein Society · 2023Article
- Genomic characterization of rare earth binding by Shewanella oneidensis.Scientific reports · 2023Article
- Evidence that the catalytic mechanism of heme a synthase involves the formation of a carbocation stabilized by a conserved glutamate.Archives of biochemistry and biophysics · 2023Article
- Elongated Riboflavin-Producing Shewanella oneidensis in a Hybrid Biofilm Boosts Extracellular Electron Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA fromMicroorganisms · 2022Article
- Recombineering in Non-Model Bacteria.Current protocols · 2022Article
- Synthetic Biology Toolbox, Including a Single-Plasmid CRISPR-Cas9 System to Biologically Engineer the Electrogenic, Metal-Resistant BacteriumACS synthetic biology · 2022Article
- Engineering Biological Electron Transfer and Redox Pathways for Nanoparticle Synthesis.Bioelectricity · 2021Article
- Engineering Cooperation in an Anaerobic Coculture.Applied and environmental microbiology · 2021Article
- Regulation of colony morphology and biofilm formation in Shewanella algae.Microbial biotechnology · 2021Article
- CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering.Nature biotechnology · 2021Article
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- Recombineering and MAGE.Nature reviews. Methods primers · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Shewanella oneidensis MR-1 is an invaluable host for the discovery and engineering of pathways important for bioremediation of toxic and radioactive metals and understanding extracellular electron transfer. However, genetic manipulation is challenging due to the lack of genetic tools. Previously, the only reliable method used for introducing DNA into Shewanella spp. at high efficiency was bacterial conjugation, enabling transposon mutagenesis and targeted knockouts using suicide vectors for gene disruptions. Here, we describe development of a robust and simple electroporation method in S. oneidensis that allows an efficiency of ~4.0 x 10
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