Evidence map›Paper›PMID 30630408›Full record

ArticleBMC evolutionary biology2019

Islands and hybrid zones: combining the knowledge from "Natural Laboratories" to explain phylogeographic patterns of the European brown hare.

Themistoklis Giannoulis, Dimitrios Plageras, Costas Stamatis, Eleni Chatzivagia, Andreas Tsipourlianos, Periklis Birtsas, Charalambos Billinis, Franz Suchentrunk, Zissis Mamuris

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC evolutionary biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Coats of Variable Hue in Tunisian Hares (GenusAnimals : an open access journal from MDPI · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Themistoklis GiannoulisLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece.
Dimitrios PlagerasLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece.
Costas StamatisLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece.
Eleni ChatzivagiaLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece.
Andreas TsipourlianosLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece.
Periklis BirtsasDepartment of Forestry and Natural Environment Administration, TEI of Thessaly, Larissa, Greece.
Charalambos BillinisFaculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
Franz SuchentrunkResearch Institute of Wildlife Ecology, University of Veterinary Medicine Vienna, Vienna, Austria.
Zissis MamurisLaboratory of Genetics, Comparative and Evolutionary Biology, University of Thessaly, Viopolis, Mezourlo, Larissa, Greece. zmamuris@gmail.com.
University of Thessaly · GRTechnological Educational Institute of Thessaly · GRUniversity of Veterinary Medicine Vienna · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim of the study was to use hybrid populations as well as island populations of the European brown hare (Lepus europaeus) to explore the effect of evolutionary events, such as the post-deglaciation translocations, spontaneous and human-mediated, local adaptation and the genetic drift in the shaping of the phylogeographic patterns of the species. For this purpose, we used molecular markers, both nuclear and mitochondrial, that are indicative for local adaptation as well as neutral markers to elucidate the patterns of population differentiation based on geographic isolation and the clade of origin. To broaden our analysis, we included data from our previous studies concerning mainland populations, to explore the genetic differentiation in the base of the geographic origin (mainland/island) of the populations.

resultsOur results suggest that local adaptation shapes the differentiation in both genomes, favoring specific alleles in nuclear genes (e.g. DQA) or haplotypes in mtDNA (e.g. Control Region, CR). mtDNA variation was found to be in a higher level and was able to give a phylogeographic signal for the populations. Furthermore, the degree of variation was influenced not only by the geographic origin, but also by the clade of origin, since specific island populations of Anatolian origin showed a greater degree of variation compared to specific mainland populations of the European clade. Concerning the hybrid population, we confirmed the existence of both clades in the territory and we provided a possible explanation for the lack of introgression between the clades.

conclusionOur results indicate that the Quaternary's climatic oscillations played a major role in the shaping of the phylogeographic patterns of the species, by isolating populations in the distinct refugia, where they adapted and differentiate in allopatry, leading to genome incompatibilities observed nowadays.

Indexed as

Hybridization, GeneticIslandsPhylogeographyAllelesAnimalsDNA, MitochondrialExonsGene FrequencyGenetic VariationHaplotypesHaresMajor Histocompatibility ComplexMicrosatellite RepeatsMitochondriaPhylogenyDNA, MitochondrialAdaptive variationBrown hareNeutral lociPhylogeography

Identifiers

PMID30630408
PMCPMC6329171
OpenAlexW2918658649

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.