Evidence map›Paper›PMID 30629954›Full record

ArticleDevelopmental biology2019

Quantitative analysis reveals genotype- and domain- specific differences between mRNA and protein expression of segmentation genes in Drosophila.

Svetlana Surkova, Alena Sokolkova, Konstantin Kozlov, Sergey V Nuzhdin, Maria Samsonova

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Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Svetlana SurkovaPeter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, St. Petersburg 195251, Russia. Electronic address: surkova_syu@spbstu.ru.
Alena SokolkovaPeter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, St. Petersburg 195251, Russia.
Konstantin KozlovPeter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, St. Petersburg 195251, Russia.
Sergey V NuzhdinPeter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, St. Petersburg 195251, Russia; Section of Molecular and Computational Biology, University of Southern California, Los Angeles 90089, CA, USA.
Maria SamsonovaPeter the Great St. Petersburg Polytechnic University, Polytechnicheskaya, 29, St. Petersburg 195251, Russia. Electronic address: m.samsonova@spbstu.ru.

Funding

Multi-scale modeling of genetic variation in a developmental networkU01GM103804 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI DEPACE, ANGELA H, MARJORAM, PAUL · 2013 to 2016
$2.0M
Allele Specific Regulation of Context Specific GRNR01GM128193 · NIGMS · UNIVERSITY OF FLORIDA · PI MCINTYRE, LAUREN M. · 2018 to 2021
$1.5M
NIGMS NIH HHS R01 GM128193NIGMS NIH HHS U01 GM103804
6 · The paper itself

Abstract

In many biological systems gene expression at mRNA and protein levels is not identical. Rigorous comparison of such differences on a spatio-temporal scale is still not feasible by high-throughput transcriptomic and proteomic analyses of early embryo development. Here, we characterize differences between mRNA and protein expression of Drosophila segmentation genes at the level of individual gene expression domains. We obtained quantitative imaging data on expression of gap genes gt and hb and pair-rule gene eve for Drosophila wild type embryos, Kr null mutants and Kr+/Kr- heterozygotes. To compare mRNA and protein expression we use several criteria including difference in amplitude and positions of expression domains, pattern shape and positional variability. For a number of gene expression domains we show examples where protein expression does not repeat mRNA expression even after a temporal delay. We calculated time delays between eve pattern formation at the level of mRNA and protein for wild type embryos, Kr mutants and Kr+/Kr- heterozygotes. We detect that in wild type embryos, the amplitudes of eve stripes 3 and 7 do not differ significantly at the level of mRNA, however, stripe 3 is higher than stripe 7 at the protein level. We further show that hb mRNA and protein expression in both anterior and posterior domains significantly differs at specific time points. The formation of hb PS4 stripe at the mRNA level proceeds five times faster than at the level of protein. With regard to spatial expression, we show that the offset between posterior gt mRNA and protein domains is much larger in Kr mutants than in wild type embryos and heterozygotes. Finally, we analyze differences in positional variability of eve stripe 7 expression in Kr mutants and Kr+/Kr- heterozygotes at the level of mRNA and protein. These results enable further perspectives to uncover mechanisms underlying discrepancies between mRNA and protein expression in early embryo.

Indexed as

GenotypeRNA, MessengerAnimalsBody PatterningDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianGene Expression Regulation, DevelopmentalHomeodomain ProteinsKruppel-Like Transcription FactorsMicroscopy, ConfocalTranscription FactorsDrosophila Proteinseve protein, DrosophilaHomeodomain ProteinsKr protein, DrosophilaKruppel-Like Transcription FactorsRNA, MessengerTranscription FactorsConfocal microscopyDiscrepancies between mRNA and protein levelsDrosophila embryoGene expressionmRNA and protein expressionPattern formationPositional informationSegmentation genes

Identifiers

PMID30629954
PMCPMC6477536

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.