ArticleBiotechnology progress2019
Pilot-scale process for magnetic bead purification of antibodies directly from non-clarified CHO cell culture.
Article in Biotechnology progress, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Robust, High-Titer, Semi-Automated, and In-Culture Antibody-Capturing Transient CHO Platform Technology.Antibodies (Basel, Switzerland) · 2025Article
- Immobilizing calcium-dependent affinity ligand onto iron oxide nanoparticles for mild magnetic mAb separation.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- Stable and reusable calcium-responsive biopolymer for affinity precipitation of therapeutic antibodies.Protein science : a publication of the Protein Society · 2024Article
- Direct Affinity Ligand Immobilization onto Bare Iron Oxide Nanoparticles Enables Efficient Magnetic Separation of Antibodies.ACS applied bio materials · 2024Article
- Combined clarification and affinity capture using magnetic resin enables efficient separation of rAAV5 from cell lysate.Molecular therapy. Methods & clinical development · 2023Article
- Molecular engineering of cyclic azobenzene-peptide hybrid ligands for the purification of human blood Factor VIII via photo-affinity chromatography.Advanced functional materials · 2023Article
- Magnetic Nanoparticles for Protein Separation and Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads.Frontiers in plant science · 2023Article
- Immobilization of EversaMolecules (Basel, Switzerland) · 2021Article
- First comprehensive view on a magnetic separation based protein purification processes: From process development to cleaning validation of a GMP-ready magnetic separator.Engineering in life sciences · 2019Article
- Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum binding capacity of 65 mg/mL and an adsorption capacity of 25-42 mg IgG/mL bead in suspension for an IgG concentration of 1 to 8 g/L. Pilot-scale separation was initially tested in a mAb capture step from 26 L clarified harvest. Small-scale experiments showed that similar mAb adsorptions were obtained in cell broth containing 40 × 10
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