ArticlePloS one2019
Cultivation-success of rare soil bacteria is not influenced by incubation time and growth medium.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- The Influence of Agroecological Intensification on Dominant and Rare Microbial Communities Across Diverse European Countries.Microbial ecology · 2025Article
- Strategies for Actinobacteria Isolation, Cultivation, and Metabolite Production that Are Biologically Important.ACS omega · 2025Review
- Synthetic communities as a model for determining interactions between a biofertilizer chassis organism and native microbial consortia.The ISME journal · 2025Article
- The soil microbiome modulates the sorghum root metabolome and cellular traits with a concomitant reduction of Striga infection.Cell reports · 2024Article
- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- Culture-Dependent and Metabarcoding Characterization of the Sugar Beet (Microorganisms · 2023Article
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- Prokaryotes of renowned Karlovy Vary (Carlsbad) thermal springs: phylogenetic and cultivation analysis.Environmental microbiome · 2022Article
- Metabolites of soil microorganisms modulate amyloid β production in Alzheimer's neurons.Scientific reports · 2022Article
- Renewed interests in the discovery of bioactive actinomycete metabolites driven by emerging technologies.Journal of applied microbiology · 2022Review
- Iron-dependent mutualism betweenISME communications · 2022Article
- Petroleum contamination significantly changes soil microbial communities in three oilfield locations in Delta State, Nigeria.Environmental science and pollution research international · 2021Article
- Latex Metabolome of Euphorbia Species: Geographical and Inter-Species Variation and its Proposed Role in Plant Defense against Herbivores and Pathogens.Journal of chemical ecology · 2021Article
- HPTLC-Based Chemical Profiling: An Approach to Monitor Plant Metabolic Expansion Caused by Fungal Endophytes.Metabolites · 2021Article
- Diversity of Culturable Bacteria Isolated from Highland Barley Cultivation Soil in Qamdo, Tibet Autonomous Region.Polish journal of microbiology · 2021Article
- Expanding the Diversity of Bacterioplankton Isolates and Modeling Isolation Efficacy with Large-Scale Dilution-to-Extinction Cultivation.Applied and environmental microbiology · 2020Article
- Dormancy dampens the microbial distance-decay relationship.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Proof of Concept of Culturomics Use of Time of Care.Frontiers in cellular and infection microbiology · 2020Article
- An approach to increase the success rate of cultivation of soil bacteria based on fluorescence-activated cell sorting.PloS one · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rare bacterial species have recently attracted interest due to their many potential beneficial functions. However, only little is known about their cultivability. In this study we test the hypotheses that the use of flow cell-sorting for cultivation results in a high proportion of rare soil bacterial isolates relative to bacterial taxa that are abundant in soil. Moreover, we investigate whether different oligotrophic cultivation media and a prolonged incubation time increase the number of cultivated rare species. In a cultivation study we used flow cell sorting to select for small cells and to separate single cells, and grew bacteria on different oligotrophic media with prolonged incubation times. The abundance of the isolates in the field was assessed by comparing them to a 454-sequencing dataset from the same soil. Consequentially, all bacterial isolates were classified as either rare (<0.01% relative abundance) or abundant (>0.01% relative abundance) in the field soil. We found more bacterial taxa among the isolates that were abundant in soil than would be expected by the proportion of abundant species in the field. Neither incubation time nor growth medium had an influence on the recovery of rare species. However, we did find differences in time until visible growth on the plate between different phylogenetic classes of the isolates. These results indicate that rare cultivable species are active and not more likely to be dormant than abundant species, as has been suggested as a reason for their rarity. Moreover, future studies should be aware of the influence incubation time might have on the phylogenetic composition of the isolate collection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.