ArticlePlant biotechnology journal2019
The basic helix-loop-helix transcription factor, OsPIL15, regulates grain size via directly targeting a purine permease gene OsPUP7 in rice.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 114 citations in OpenAlex.
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- Multiplex Gene Editing and Effect Analysis of Yield, Fragrance, and Blast Resistance Genes in Rice.Genes · 2026Article
- Integrated Multi-Omics Reveals the Molecular Basis Underlying Wheat Grain Development and IdentifiesPlants (Basel, Switzerland) · 2025Article
- The bHLH transcription factor PIL1 orchestrates starch synthesis in key cereal crops.Nature communications · 2025Article
- The Phytochrome-Interacting Factor OsPIL11 Coordinates Grain Weight and Grain Number Via Directly Regulating the Expression of OsMIR530 and OsCKX2 in Rice.Rice (New York, N.Y.) · 2025Article
- bHLH Transcription Factors in Cereal Crops: Diverse Functions in Regulating Growth, Development and Stress Responses.International journal of molecular sciences · 2025Review
- The SmWRKY32-SmbHLH65/SmbHLH85 regulatory module mediates tanshinone biosynthesis inHorticulture research · 2025Article
- Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli.The Plant cell · 2025Article
- Rice grain size: current regulatory mechanisms and future perspectives.Journal of plant research · 2025Review
- WRKY36-PIL15 Transcription Factor Complex Negatively Regulates Sheath Blight Resistance and Seed Development in Rice.Plants (Basel, Switzerland) · 2025Article
- A bHLH transcription factor RrUNE12 regulates salt tolerance and promotes ascorbate synthesis.Plant cell reports · 2025Article
- The E3 ligase OsPUB33 controls rice grain size and weight by regulating the OsNAC120-BG1 module.The Plant cell · 2024Article
- Molecular mechanisms controlling grain size and weight and their biotechnological breeding applications in maize and other cereal crops.Journal of advanced research · 2024Review
- The phytochrome-interacting factor genes PIF1 and PIF4 are functionally diversified due to divergence of promoters and proteins.The Plant cell · 2024Article
- Mapping and candidate gene analysis of QTLs for grain shape in a rice chromosome segment substitution line Z485 and breeding of SSSLs.Molecular breeding : new strategies in plant improvement · 2024Article
- Article
- Rice PIFs: Critical regulators in rice development and stress response.Plant molecular biology · 2024Review
- Genetic Basis of Grain Size and Weight in Rice, Wheat, and Barley.International journal of molecular sciences · 2023Review
- Sugar starvation activates the OsSnRK1a-OsbHLH111/OsSGI1-OsTPP7 module to mediate growth inhibition of rice.Plant biotechnology journal · 2023Article
- TaMADS29 interacts with TaNF-YB1 to synergistically regulate early grain development in bread wheat.Science China. Life sciences · 2023Article
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Authors and funding
9 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
As members of the basic helix-loop-helix transcription factor families, phytochrome-interacting factors regulate an array of developmental responses ranging from seed germination to plant growth. However, little is known about their roles in modulating grain development. Here, we firstly analyzed the expression pattern of rice OsPIL genes in grains and found that OsPIL15 may play an important role in grain development. We then generated knockout (KO) OsPIL15 lines in rice using CRISPR/Cas9 technology, the silencing expression of OsPIL15 led to increased numbers of cells, which thus enhanced grain size and weight. Moreover, overexpression and suppression of OsPIL15 in the rice endosperm resulted in brown rice showing grain sizes and weights that were decreased and increased respectively. Further studies indicated that OsPIL15 binds to N1-box (CACGCG) motifs of the purine permease gene OsPUP7 promoter. Measurement of isopentenyl adenosine, a bioactive form of cytokinin (CTK), revealed increased contents in the OsPIL15-KO spikelets compared with the wild-type. Overall, our results demonstrate a possible pathway whereby OsPIL15 directly targets OsPUP7, affecting CTK transport and thereby influencing cell division and subsequent grain size. These findings provide a valuable insight into the molecular functions of OsPIL15 in rice grains, highlighting a useful genetic improvement leading to increased rice yield.
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