ArticleJournal of assisted reproduction and genetics2019
Cumulus-corona gene expression analysis combined with morphological embryo scoring in single embryo transfer cycles increases live birth after fresh transfer and decreases time to pregnancy.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Improved clinical outcomes after non-invasive oocyte selection and Day 3 eSET in ICSI patients.Reproductive biology and endocrinology : RB&E · 2021Trial
- Prediction of high-quality human oocytes and embryos through the analysis of gene expression in cumulus cells.Journal of ovarian research · 2025Review
- Live birth in patients stimulated with r-hFSH or r-hFSH: r-hLH is strongly associated with cumulus cell derived gene expression models.Reproductive biology and endocrinology : RB&E · 2025Observational
- Implication of the Vaginal Microbiome in Female Infertility and Assisted Conception Outcomes.Genomics, proteomics & bioinformatics · 2025Article
- The relationship between vaginal and endometrial microbiota and the outcome of Frozen-Thawed embryo transfer during hormone replacement cycles.BMC microbiology · 2025Article
- Transcriptional Changes in Cumulus Cells During In Vitro Maturation of Human Oocytes.Molecular reproduction and development · 2025Article
- Bioinformatic Analysis of Human Cumulus Cells to Unravel Cellular's Processes that Could Be Used to Establish Oocyte Quality Biomarkers with Clinical Application.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Blastocyst versus cleavage transfers: who benefits?Archives of gynecology and obstetrics · 2022Article
- Clinical and Molecular Effects of GnRH Agonist and Antagonist on The Cumulus Cells in TheInternational journal of fertility & sterility · 2021Article
- The Disordered Vaginal Microbiota Is a Potential Indicator for a Higher Failure ofFrontiers in medicine · 2020Article
- Apoptosis of mural granulosa cells is increased in women with diminished ovarian reserve.Journal of assisted reproduction and genetics · 2019Article
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Abstract
purposeClinical pregnancy rate after IVF with eSET stagnates between 30 and 40%. In order to increase pregnancy and live birth rates, multiple embryo transfer is still common practice. Providing additional non-invasive tools to choose the competent embryo for transfer could avoid multiple pregnancy and improve time to pregnancy. Cumulus mRNA analysis with quantitative PCR (QPCR) is a non-invasive approach. However, so far, no gene sets have been validated in prospective interventional studies.
methodsA prospective interventional single-center pilot study with two matched controls (day-3 and day-5 eSET) was performed in 96 patients consenting to the analysis of the cumulus-corona of their oocytes. All patients were super-ovulated for ICSI and eSET at day 3. All oocytes were denuded individually and cumulus was analyzed by quantitative PCR using three predictive genes (EFNB2, SASH1, CAMK1D) and two housekeeping genes (UBC and β2M). Patients (n = 62) with 2 or more day-3 embryos (good or excellent morphology) had their embryo chosen following the normalized expression of the genes.
resultsCorona testing significantly increased the clinical pregnancy and live births rates (63% and 55%) compared to single embryo transfer (eSET) on day 3 (27% and 23%: p < 0.001) and day 5 (43% and 39%: p = 0.022 and p = 0.050) fresh transfer cycle controls with morphology-only selection. Time-to-pregnancy was significantly reduced, regardless of the number of good-quality embryos available on day 3.
conclusionCombining standard morphology scoring and cumulus/corona gene expression analysis increases day-3 eSET results and significantly reduces the time to pregnancy. TRIAL REGISTRATION NUMBER: This is not an RCT study and was only registered by the ethical committee of the University Hospital UZBRUSSEL of the Vrije Universiteit Brussel VUB (BUN: 143201318000).
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