ArticleMikrochimica acta2019
Phytic acid functionalized Fe
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Sustainable Phosphorylated Cellulose Nanocrystals: A Dual-Affinity Platform for High-Efficiency Enrichment of Intact Glycopeptides and Phosphopeptides.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- ATP-Coated Dual-Functionalized Titanium(IV) IMAC Material for Simultaneous Enrichment and Separation of Glycopeptides and Phosphopeptides.Journal of proteome research · 2023Article
- Targeted immobilization of titanium (IV) on magnetic mesoporous nanomaterials derived from metal-organic frameworks for high-efficiency phosphopeptide enrichment in biological samples.Mikrochimica acta · 2020Article
- Specific enrichment of phosphopeptides by using magnetic nanocomposites of type FeMikrochimica acta · 2020Article
- A polymer monolith composed of a perovskite and cucurbit[6]uril hybrid for highly selective enrichment of phosphopeptides prior to mass spectrometric analysis.Mikrochimica acta · 2019Article
- Binary magnetic metal-organic frameworks composites: a promising affinity probe for highly selective and rapid enrichment of mono- and multi-phosphopeptides.Mikrochimica acta · 2019Article
- Titanium(IV)-functionalized zirconium-organic frameworks as dual-metal affinity probe for recognition of endogenous phosphopeptides prior to mass spectrometric quantification.Mikrochimica acta · 2019Article
- Nanoparticle-based surface assisted laser desorption ionization mass spectrometry: a review.Mikrochimica acta · 2019Article
- Preparation of a hydrophilic interaction liquid chromatography material by sequential electrostatic deposition of layers of polyethyleneimine and hyaluronic acid for enrichment of glycopeptides.Mikrochimica acta · 2019Article
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Authors and funding
8 authors.
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Abstract
A novel magnetic nanomaterial for use in metal ion based affinity chromatography is described. It is based on the chelation between the phosphate groups of phytic acid (PA) and Ti(IV) ions. Due to the large number (6) of phosphate groups of PA, it has a large capacity for Ti(IV) ions. PA was first immobilized on magnetite nanoparticles (PA-MNPs) and then loaded with Ti(IV) ions to obtain the sorbent (Ti-PA-MNPs). The fraction of Ti(IV) ions on the surface of PA-MNPs that is exposed to the solution binds the phosphate groups of phosphopeptides. The bound phosphopeptides can then be magnetically separated. The method was applied to the enrichment of the phosphopeptides in a β-casein tryptic digest. A tryptic digest of bovine serum albumin (BSA) was added at a molar ratio (β-casein to BSA) of 1:2000 to study selectivity. The phosphopeptides were quantified by mass spectrometry. The limit of detection can be as low as 8 × 10
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