ArticleeNeuro
High-Fidelity Imaging in Brain-Wide Structural Studies Using Light-Sheet Microscopy.
Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.iScience · 2026Article
- Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.Biomedical optics express · 2026Article
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.Communications biology · 2025Article
- Early life psychosocial stress increases binge-like ethanol consumption and CSF1R inhibition prevents stress-induced alterations in microglia and brain macrophage population density.Brain, behavior, & immunity - health · 2025Article
- Deep learning-based vessel extraction in 3D confocal microscope images of cleared human glioma tissues.Biomedical optics express · 2024Article
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
- Advances in studying whole mouse brain vasculature using high-resolution 3D light microscopy imaging.Neurophotonics · 2022Review
- Benchmarking of deep learning algorithms for 3D instance segmentation of confocal image datasets.PLoS computational biology · 2022Review
- Seeing the Forest and Its Trees Together: Implementing 3D Light Microscopy Pipelines for Cell Type Mapping in the Mouse Brain.Frontiers in neuroanatomy · 2021Review
- Innovative high-resolution microCT imaging of animal brain vasculature.Brain structure & function · 2020Article
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
- Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes.Frontiers in neuroscience · 2020Review
- Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy.Frontiers in cellular neuroscience · 2018Article
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Authors and funding
7 authors.
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Abstract
Light-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution and, in combination with tissue clearing methods, is often employed to reconstruct the cyto-architecture over the intact mouse brain. Inherently to LSM, however, residual opaque objects, always present to some extent even in extremely well optically cleared samples, cause stripe artifacts, which, in the best case, severely affect image homogeneity and, in the worst case, completely obscure features of interest. Here, demonstrating two example applications in intact optically cleared mouse brains, we report how Bessel beams reduce streaking artifacts and produce high-fidelity structural data for the brain-wide morphology of neuronal and vascular networks. We found that a third of the imaged volume of the brain was affected by strong striated image intensity inhomogeneity and, furthermore, a significant amount of information content lost with Gaussian illumination was accessible when interrogated with Bessel beams. In conclusion, Bessel beams produce high-fidelity structural data of improved image homogeneity and might significantly relax demands placed on the automated tools to count, trace, or segment fluorescent features of interest.
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