Evidence map›Paper›PMID 30627058›Full record

ArticleContrast media & molecular imaging2018

Multicolour In Vivo Bioluminescence Imaging Using a NanoLuc-Based BRET Reporter in Combination with Firefly Luciferase.

Arthur Taylor, Jack Sharkey, Antonius Plagge, Bettina Wilm, Patricia Murray

Open access · hybridAbstract read
In one paragraph

Article in Contrast media & molecular imaging, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 38 citations in OpenAlex.

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  3. OptimizediScience · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Arthur TaylorDepartment of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK.ORCID 0000-0003-2028-6694
Jack SharkeyDepartment of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK.ORCID 0000-0002-0516-7622
Antonius PlaggeDepartment of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK.
Bettina WilmDepartment of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK.
Patricia MurrayDepartment of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK.
University of Liverpool · GB

Funding

Biotechnology and Biological Sciences Research CouncilMedical Research Council MR/K026739/1Medical Research Council MR/L012707/1
6 · The paper itself

Abstract

The ability to track the biodistribution and fate of multiple cell populations administered to rodents has the potential to facilitate the understanding of biological processes in a range of fields including regenerative medicine, oncology, and host/pathogen interactions. Bioluminescence imaging is an important tool for achieving this goal, but current protocols rely on systems that have poor sensitivity or require spectral decomposition. Here, we show that a bioluminescence resonance energy transfer reporter (BRET) based on NanoLuc and LSSmOrange in combination with firefly luciferase enables the unambiguous discrimination of two cell populations

Indexed as

AnimalsBioluminescence Resonance Energy Transfer TechniquesCell LineGenes, ReporterGenetic VectorsLuciferases, FireflyLuminescent MeasurementsMiceMice, Inbred BALB COptical ImagingRAW 264.7 CellsTissue DistributionLuciferases, Firefly

Identifiers

PMID30627058
PMCPMC6305057
OpenAlexW2902870924

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.