ArticleMicrobial cell factories2019
Improvement of isoprene production in Escherichia coli by rational optimization of RBSs and key enzymes screening.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 36 citations in OpenAlex.
- Enhancement of levopimaradiene production inChinese herbal medicines · 2026Article
- Isolation and identification of Alcaligenes faecalis W2-3 with high-yield production of dimethyl disulfide.Scientific reports · 2025Article
- Optimization of IspSApplied and environmental microbiology · 2023Article
- Multi-level metabolic engineering of Escherichia coli for high-titer biosynthesis of 2'-fucosyllactose and 3-fucosyllactose.Microbial biotechnology · 2022Article
- MICROBIAL isoprene production: an overview.World journal of microbiology & biotechnology · 2022Review
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Solvent-Induced Selectivity of Isoprene From Bio-Derived Prenol.Frontiers in chemistry · 2022Article
- Biodegradation of aromatic pollutants meets synthetic biology.Synthetic and systems biotechnology · 2021Article
- Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAs an essential platform chemical mostly used for rubber synthesis, isoprene is produced in industry through chemical methods, derived from petroleum. As an alternative, bio-production of isoprene has attracted much attention in recent years. Previous researches were mostly focused on key enzymes to improve isoprene production. In this research, besides screening of key enzymes, we also paid attention to expression intensity of non-key enzymes.
resultsFirstly, screening of key enzymes, IDI, MK and IspS, from other organisms and then RBS optimization of the key enzymes were carried out. The strain utilized IDI
conclusionsThis study prove that desired enzyme expression and increased isoprene production were obtained after RBS sequence optimization. RBS optimization of genes could be a powerful strategy for metabolic engineering of strain. Moreover, to increase the production of engineered strain, attention should not only be focused on the key enzymes, but also on the non-key enzymes.
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