ArticlePloS one2019
Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 169 citations in OpenAlex.
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- Endogenous RALF peptide function is required for powdery mildew host colonization.The New phytologist · 2026Article
- A Rab/Kinesin-12/kinase module couples vesicle delivery and phragmoplast dynamics during plant cell cytokinesis.The EMBO journal · 2026Article
- Optimized R2 retroelement complexes for DNA insertion into plant genomes.Nature biotechnology · 2026Article
- Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunity.Nature plants · 2026Article
- Reduced Cas9 transgene silencing by incorporation of intron sequences.Nature communications · 2025Article
- Chitin Soil Amendment Triggers Systemic Plant Disease Resistance Through Enhanced Pattern-Triggered Immunity.Plant biotechnology journal · 2025Article
- High-throughput genetic transformation and genome editing in pearl millet (Pennisetum glaucum L.).Plant physiology · 2025Article
- CEPR2 perceives group II CEPs to regulate cell surface receptor-mediated immunity in Arabidopsis.PLoS pathogens · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- Berberine bridge enzyme-like oxidases orchestrate homeostasis and signaling of oligogalacturonides in defense and upon mechanical damage.The Plant journal : for cell and molecular biology · 2025Article
- Isolation and transformation of perennial ryegrass (Frontiers in plant science · 2025Article
- CEP signaling coordinates plant immunity with nitrogen status.Nature communications · 2024Article
- A peptide-receptor module links cell wall integrity sensing to pattern-triggered immunity.Nature plants · 2024Article
- An efficient multiplex approach to CRISPR/Cas9 gene editing in citrus.Plant methods · 2024Article
- An optimised CRISPR Cas9 and Cas12a mutagenesis toolkit for Barley and Wheat.Plant methods · 2024Article
- Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene.Transgenic research · 2024Article
- New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i.Plant methods · 2024Article
- CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in pigeonpea and groundnut.Functional & integrative genomics · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison of components and T-DNA architectures for CRISPR-mediated gene editing in Arabidopsis, testing multiple promoters, terminators, sgRNA backbones and Cas9 alleles. We identified a T-DNA architecture that usually results in stable (i.e. homozygous) mutations in the first generation after transformation. Notably, the transcription of sgRNA and Cas9 in head-to-head divergent orientation usually resulted in highly active lines. Our Arabidopsis data may prove useful for optimization of CRISPR methods in other plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.