ArticlePLoS genetics2019
Allele-specific RNA imaging shows that allelic imbalances can arise in tissues through transcriptional bursting.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Multi-layered dosage compensation of the avian Z chromosome by increased transcriptional burst frequency and elevated translational rates.Nature communications · 2025Article
- Engineered transcription-associated Cas9 targeting in eukaryotic cells.Nature communications · 2024Article
- Engineered transcription-associated Cas9 targeting in eukaryotic cells.bioRxiv : the preprint server for biology · 2024Article
- Prevalence of and gene regulatory constraints on transcriptional adaptation in single cells.Genome biology · 2024Article
- Prevalence of and gene regulatory constraints on transcriptional adaptation in single cells.bioRxiv : the preprint server for biology · 2023Article
- The transcriptional legacy of developmental stochasticity.Nature communications · 2023Article
- Article
- Allelic correlation is a marker of trade-offs between barriers to transmission of expression variability and signal responsiveness in genetic networks.Cell systems · 2022Article
- Simultaneous brain cell type and lineage determined by scRNA-seq reveals stereotyped cortical development.Cell systems · 2022Article
- The stochastic nature of genome organization and function.Current opinion in genetics & development · 2022Review
- Transcriptional bursts and heterogeneity among cardiomyocytes in hypertrophic cardiomyopathy.Frontiers in cardiovascular medicine · 2022Article
- Noise suppression in stochastic genetic circuits using PID controllers.PLoS computational biology · 2021Article
- Transcriptional bursts explain autosomal random monoallelic expression and affect allelic imbalance.PLoS computational biology · 2021Article
- Perspective: Controlling Epidermal Terminal Differentiation with Transcriptional Bursting and RNA Bodies.Journal of developmental biology · 2020Review
- Review
- Stochastic allelic expression as trigger for contractile imbalance in hypertrophic cardiomyopathy.Biophysical reviews · 2020Review
- In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription.Nature biotechnology · 2020Article
- New Technologies to Image Tumors.Cancer treatment and research · 2020Article
- New subtypes of allele-specific epigenetic effects: implications for brain development, function and disease.Current opinion in neurobiology · 2019Review
- Single cell transcriptome in aneuploidies reveals mechanisms of gene dosage imbalance.Nature communications · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Extensive cell-to-cell variation exists even among putatively identical cells, and there is great interest in understanding how the properties of transcription relate to this heterogeneity. Differential expression from the two gene copies in diploid cells could potentially contribute, yet our ability to measure from which gene copy individual RNAs originated remains limited, particularly in the context of tissues. Here, we demonstrate quantitative, single molecule allele-specific RNA FISH adapted for use on tissue sections, allowing us to determine the chromosome of origin of individual RNA molecules in formaldehyde-fixed tissues. We used this method to visualize the allele-specific expression of Xist and multiple autosomal genes in mouse kidney. By combining these data with mathematical modeling, we evaluated models for allele-specific heterogeneity, in particular demonstrating that apparent expression from only one of the alleles in single cells can arise as a consequence of low-level mRNA abundance and transcriptional bursting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.