ArticlePloS one2019
Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Distinctive domestication of farmhouse beer yeasts preserved pre-industrial genetic and phenotypic diversity.Current biology : CB · 2026Article
- Exploring CRISPR/Cas9-Mediated Gene Editing Advances in Conventional and Non-conventional Yeast Species.Applied biochemistry and biotechnology · 2025Review
- Understanding brewing trait inheritance inmSystems · 2024Article
- New Genomic Techniques applied to food cultures: a powerful contribution to innovative, safe, and sustainable food products.FEMS microbiology letters · 2024Article
- Study of novel androgen receptor V770 variant in androgen insensitivity syndrome patients reveals the transitional state of the androgen receptor ligand binding domain homodimer.Protein science : a publication of the Protein Society · 2023Article
- CRISPR-Cas9 engineering in the hybrid yeast Zygosaccharomyces parabailii can lead to loss of heterozygosity in target chromosomes.FEMS yeast research · 2023Article
- A Saccharomyces eubayanus haploid resource for research studies.Scientific reports · 2022Article
- Toolbox for the structure-guided evolution of ferulic acid decarboxylase (FDC).Scientific reports · 2022Article
- Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switching.Applied microbiology and biotechnology · 2021Article
- Industrially ApplicableApplied and environmental microbiology · 2021Article
- Proteomics Answers Which Yeast Genes Are Specific for Baking, Brewing, and Ethanol Production.Bioengineering (Basel, Switzerland) · 2020Article
- Metabolic Engineering of Wine Strains ofGenes · 2020Review
- Into the wild: new yeast genomes from natural environments and new tools for their analysis.FEMS yeast research · 2020Review
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Overexpression ofMicroorganisms · 2019Article
- Multiplex genome editing of microorganisms using CRISPR-Cas.FEMS microbiology letters · 2019Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Today's beer market is challenged by a decreasing consumption of traditional beer styles and an increasing consumption of specialty beers. In particular, lager-type beers (pilsner), characterized by their refreshing and unique aroma and taste, yet very uniform, struggle with their sales. The development of novel variants of the common lager yeast, the interspecific hybrid Saccharomyces pastorianus, has been proposed as a possible solution to address the need of product diversification in lager beers. Previous efforts to generate new lager yeasts through hybridization of the ancestral parental species (S. cerevisiae and S. eubayanus) yielded strains with an aromatic profile distinct from the natural biodiversity. Unfortunately, next to the desired properties, these novel yeasts also inherited unwanted characteristics. Most notably is their phenolic off-flavor (POF) production, which hampers their direct application in the industrial production processes. Here, we describe a CRISPR-based gene editing strategy that allows the systematic and meticulous introduction of a natural occurring mutation in the FDC1 gene of genetically complex industrial S. cerevisiae strains, S. eubayanus yeasts and interspecific hybrids. The resulting cisgenic POF- variants show great potential for industrial application and diversifying the current lager beer portfolio.
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