ArticleAnalytical chemistry2019
Simultaneous Single-Channel Multiplexing and Quantification of Carbapenem-Resistant Genes Using Multidimensional Standard Curves.
Article in Analytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Application of Universal LNA Probe-Mediated Multiplex Real-Time PCR for the Detection of Antibiotic Resistance Genes.Molecular biotechnology · 2026Article
- Smart-Plexer: a breakthrough workflow for hybrid development of multiplex PCR assays.Communications biology · 2023Article
- Next-generation molecular diagnostics: Leveraging digital technologies to enhance multiplexing in real-time PCR.Trends in analytical chemistry : TRAC · 2023Article
- Adaptive Filtering Framework to Remove Nonspecific and Low-Efficiency Reactions in Multiplex Digital PCR Based on Sigmoidal Trends.Analytical chemistry · 2022Article
- The potential of digital molecular diagnostics for infectious diseases in sub-Saharan Africa.PLOS digital health · 2022Review
- Single-channel digital LAMP multiplexing using amplification curve analysis.Sensors & diagnostics · 2022Article
- Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.ACS omega · 2021Article
- Coupling Machine Learning and High Throughput Multiplex Digital PCR Enables Accurate Detection of Carbapenem-Resistant Genes in Clinical Isolates.Frontiers in molecular biosciences · 2021Article
- PCR Multiplexing Based on a Single Fluorescent Channel Using Dynamic Melting Curve Analysis.ACS omega · 2020Article
- Rapid Detection of Azole-Resistant Aspergillus fumigatus in Clinical and Environmental Isolates by Use of a Lab-on-a-Chip Diagnostic System.Journal of clinical microbiology · 2020Article
- Framework for DNA Quantification and Outlier Detection Using Multidimensional Standard Curves.Analytical chemistry · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Multiplexing and quantification of nucleic acids, both have, in their own right, significant and extensive use in biomedical related fields. Currently, the ability to detect several nucleic acid targets in a single-reaction scales linearly with the number of targets; an expensive and time-consuming feat. Here, we propose a new methodology based on multidimensional standard curves that extends the use of real-time PCR data obtained by common qPCR instruments. By applying this novel methodology, we achieve simultaneous single-channel multiplexing and enhanced quantification of multiple targets using only real-time amplification data. This is obtained without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis. Given the importance and demand for tackling challenges in antimicrobial resistance, the proposed method is applied to four of the most prominent carbapenem-resistant genes: bla
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.