Evidence map›Paper›PMID 30624045›Full record

ArticleACS sensors2019

Emulsion Agglutination Assay for the Detection of Protein-Protein Interactions: An Optical Sensor for Zika Virus.

Qifan Zhang, Lukas Zeininger, Ki-Joo Sung, Eric A Miller, Kosuke Yoshinaga, Hadley D Sikes, Timothy M Swager

Open access · greenAbstract read
In one paragraph

Article in ACS sensors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
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  3. Review
  4. Review
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  11. Fluorescent Janus emulsions for biosensing ofProceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  12. Rapid Detection ofACS central science · 2019
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Qifan ZhangDepartment of Chemistry and Institute for Soldier Nanotechnologies , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Lukas ZeiningerDepartment of Chemistry and Institute for Soldier Nanotechnologies , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Ki-Joo SungDepartment of Chemical Engineering , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Eric A MillerDepartment of Chemical Engineering , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Kosuke YoshinagaDepartment of Chemistry and Institute for Soldier Nanotechnologies , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.
Hadley D SikesDepartment of Chemical Engineering , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.ORCID 0000-0002-7096-138X
Timothy M SwagerDepartment of Chemistry and Institute for Soldier Nanotechnologies , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.ORCID 0000-0002-3577-0510
Institute for Soldier Nanotechnologies · USMassachusetts Institute of Technology · US

Funding

VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI MATTHEW G. VANDER HEIDEN · 1985 to 2026
$93.9M
Radicals and Polyradicals for Dynamic Nuclear PolarizationR01GM095843 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SWAGER, TIMOTHY M · 2011 to 2018
$2.5M
NCI NIH HHS P30 CA014051NIGMS NIH HHS R01 GM095843
6 · The paper itself

Abstract

A new class of Janus emulsion agglutination assay is reported for the detection of interfacial protein-protein interactions. Janus emulsion droplets are functionalized with a thermally stable, antigen binding protein rcSso7d variant (rcSso7d-ZNS1) for the detection of Zika NS1 protein. The emulsion droplets containing fluorescent dyes in their hydrocarbon and fluorocarbon phases intensify the intrinsic optical signal with the emission intensity ratio, which can be detected by a simple optical fiber. This assay provides an opportunity for the in-field detection of Zika virus and other pathogens with a stable, inexpensive, and convenient device.

Indexed as

Acrylic ResinsAgglutinationAgglutination TestsArchaeal ProteinsDNA-Binding ProteinsEmulsionsEscherichia coliFluorescent DyesFluorometryLimit of DetectionMaleimidesPerylenePiperazinesProtein BindingPyrrolesViral Nonstructural ProteinsAcrylic ResinsArchaeal ProteinsDNA-Binding ProteinsEmulsionsFluorescent DyesMaleimidesN-(2-(4-o-fluorophenylpiperazin-1-yl)ethyl)-2,5-dimethyl-1 -phenylpyrrole-3,4-dicarboximideNS1 protein, zika virusPerylenePiperazinesPyrrolesSso7d protein, SulfolobusViral Nonstructural ProteinscarbohydratesemulsionsJanus particlesrcSso7dsensorsZika virus

Identifiers

PMID30624045
PMCPMC7255051
OpenAlexW2910147525

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.