Enhanced N-acetyl-D-neuraminic production from glycerol and N-acetyl-D-glucosamine by metabolically engineered Escherichia coli with a two-stage pH-shift control strategy.

De-Qiang Zhu, Jian-Rong Wu, Xiao-Bei Zhan, Li Zhu, Yun Jiang

Abstract read

In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.3field-weighted citation impact, top 42% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

Recent advances onSynthetic and systems biotechnology · 2023
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

De-Qiang ZhuKey Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.ORCID http://orcid.org/0000-0003-2965-0181

Jian-Rong WuKey Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Xiao-Bei ZhanKey Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. xbzhan@yahoo.com.

Li ZhuJiangsu Rayguang Biotech Co. Ltd., Wuxi, 214122, China.

Yun JiangKey Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Jiangnan University · CNShandong Academy of Sciences · CN

Funding

National Natural Science Foundation of China 31171640

6 · The paper itself

Abstract

Typical N-acetyl-D-neuraminic acid (Neu5Ac) production uses N-acetyl-D-glucosamine (GlcNAc) and excess pyruvate as substrates in the enzymatic or whole-cell biocatalysis process. In a previous study, a Neu5Ac-producing biocatalytic process via engineered Escherichia coli SA-05/pDTrc-AB/pCDF-pck-ppsA was constructed without exogenous pyruvate. In this study, glycerol was found to be a good energy source compared with glucose for the catalytic system with resting cells, and Neu5Ac production increased to 13.97 ± 0.27 g L

Indexed as

AcetylglucosamineBatch Cell Culture TechniquesBiocatalysisEscherichia coliGlycerolHydrogen-Ion ConcentrationMetabolic EngineeringMicroorganisms, Genetically-ModifiedN-Acetylneuraminic AcidPyruvic AcidAcetylglucosamineGlycerolN-Acetylneuraminic AcidPyruvic AcidBiocatalytic processEnergy sourceFed-batchGlycerolN-Acetyl-D-neuraminic acid

Identifiers

PMID30623269

DOI10.1007/s10295-018-02132-8

OpenAlexW2909374292

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.