ArticleIntensive care medicine experimental2019
Time-related changes in hepatic and colonic mitochondrial oxygen consumption after abdominal infection in rats.
Article in Intensive care medicine experimental, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 16 citations in OpenAlex.
- Milrinone and levosimendan improve microvascular perfusion in septic rats: a randomized, placebo-controlled trial.Intensive care medicine experimental · 2026Article
- Effects of Dobutamine and Vasopressin on Microcirculation and Mitochondrial Function in the Liver and Colon of Septic Rats: A Randomized Placebo-Controlled Trial.Shock (Augusta, Ga.) · 2026Article
- Remote ischemic preconditioning improves ileal microvascular oxygenation during rodent hemorrhagic shock without improving variables of microcirculation and mitochondrial respiration.Scientific reports · 2025Article
- Cytochrome bd-II oxidase CyxA promotes the pathogenicity ofVirulence · 2025Article
- Reversible impairment of non-invasively assessed mitochondrial oxygen metabolism in the long-term course of patients with sepsis: a prospective monocentric cohort study.Intensive care medicine experimental · 2025Article
- Effects of Local Vasodilators and the Autonomic Nervous System on Microcirculation and Mitochondrial Function in Septic Rats.International journal of molecular sciences · 2024Article
- Effects of remote ischemic preconditioning on early markers of intestinal injury in experimental hemorrhage in rats.Scientific reports · 2024Article
- Pravastatin Improves Colonic and Hepatic Microcirculatory Oxygenation during Sepsis without Affecting Mitochondrial Function and ROS Production in Rats.International journal of molecular sciences · 2023Article
- Review
- Tissue Damage, Not Infection, Triggers Hepatic Unfolded Protein Response in an Experimental Rat Peritonitis Model.Frontiers in medicine · 2022Article
- Article
- Sodium Thiosulfate Improves Intestinal and Hepatic Microcirculation Without Affecting Mitochondrial Function in Experimental Sepsis.Frontiers in immunology · 2021Article
- Indomethacin Increases the Efficacy of Oxygen Utilization of Colonic Mitochondria and Uncouples Hepatic Mitochondria in Tissue Homogenates From Healthy Rats.Frontiers in medicine · 2020Article
- Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEvidence suggests that early adaptive responses of hepatic mitochondria occur in experimentally induced sepsis. Little is known about both colonic mitochondrial function during abdominal infection and long-term changes in mitochondrial function under inflammatory conditions. We hypothesize that hepatic and colonic mitochondrial oxygen consumption changes time-dependently after sterile laparotomy and in the course of abdominal infection. The aim of the present study was to investigate the hepatic and colonic mitochondrial respiration after sterile laparotomy and abdominal infection over up to 96 h.
methodsAfter approval of the local Animal Care and Use Committee, 95 Wistar rats were randomized into 8 groups (n = 11-12): 1-4 sham (laparotomy only) and 5-8 colon ascendens stent peritonitis (CASP). Healthy, unoperated animals served as controls (n = 9). The mitochondrial respiration in colon and liver homogenates was assessed 24, 48, 72, and 96 h after surgery. Mitochondrial oxygen consumption was determined using a Clark-type electrode. State 2 (oxygen consumption in the presence of the substrates for complexes I and II) and state 3 respiration (ADP dependent) were assessed. The respiratory control ratio (RCR state 3/state 2) and ADP/O ratio (ADP added/oxygen consumed) were calculated for both complexes. Data are presented as means ± SD, two-way ANOVA followed by Tukey's post hoc test.
resultsHepatic RCR was initially (after 24 h) elevated in both operated groups; after 48 h only, the septic group was elevated compared to controls. In CASP groups, the hepatic ADP/O ratio for complex I was elevated after 24 h (vs. controls) and after 48 h (vs. sham) but declined after 72 h (vs. controls). The ADP/O ratio for complex II stayed unchanged over the time period until 96 h. The colonic RCR and ADP/O did not change over time after sham or CASP operation.
conclusionHepatic, but not colonic, mitochondrial respiration is increased in the initial phase (until 48 h) and normalizes in the longer course of time (until 96 h) of abdominal infection.
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