ArticleRegenerative therapy2019
Induction of functional islet-like cells from human iPS cells by suspension culture.
Article in Regenerative therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Effectiveness of human induced pluripotent stem cell-derived β-like cell/islet transplantation in animal models of diabetes: A meta-analysis of preclinical studies.Global health & medicine · 2026Review
- Evaluating iPSC-based interventions for diabetes: a systematic review and meta-analysis.Diabetology & metabolic syndrome · 2026Article
- Bioengineering Pancreatic Organoids and iPSC-Derived β-Cells for Diabetes: Materials, Devices, and Translational Challenges.Bioengineering (Basel, Switzerland) · 2026Review
- A Novel Immunodeficient Hyperglycemic Mouse Carrying the Ins1 Akita Mutation for Xenogeneic Islet Cell Transplantation.Transplantation · 2025Article
- Evaluation of Pancreatic β-cell Differentiation Efficiency of Human iPSC Lines for Clinical UseCurrent stem cell research & therapy · 2024Article
- Article
- Islets in the body are never flat: transitioning from two-dimensional (2D) monolayer culture to three-dimensional (3D) spheroid for better efficiency in the generation of functional hPSC-derived pancreatic β cells in vitro.Cell communication and signaling : CCS · 2023Review
- Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties.Cells · 2023Article
- Importance of multiple endocrine cell types in islet organoids for type 1 diabetes treatment.Translational research : the journal of laboratory and clinical medicine · 2022Review
- The cultivation conditions affect the aggregation and functionality of β-cell lines alone and in coculture with mesenchymal stromal/stem cells.Engineering in life sciences · 2022Article
- Type 1 diabetes and engineering enhanced islet transplantation.Advanced drug delivery reviews · 2022Review
- Stem cell therapy for insulin-dependent diabetes: Are we still on the road?World journal of stem cells · 2022Review
- Making human pancreatic islet organoids: Progresses on the cell origins, biomaterials and three-dimensional technologies.Theranostics · 2022Review
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- Article
- Review
- Lotus-root-shaped cell-encapsulated construct as a retrieval graft for long-term transplantation of human iPSC-derived β-cells.iScience · 2021Article
- Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions.Nature protocols · 2021Article
- Recent progress in pancreatic islet cell therapy.Inflammation and regeneration · 2021Review
- Recent Advances in the Generation of β-Cells from Induced Pluripotent Stem Cells as a Potential Cure for Diabetes Mellitus.Advances in experimental medicine and biology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionTo complement islet transplantation for type1 diabetic patients, cell-based therapy using pluripotent stem cells such as ES cells and iPS cells is promising. Many papers have already reported the induction of pancreatic β cells from these cell types, but a suspension culture system has not usually been employed. The aim of this study is to establish a suspension culture method for inducing functional islet-like cells from human iPS cells.
methodsWe used 30 ml spinner type culture vessels for human iPS cells throughout the differentiation process. Differentiated cells were analyzed by immunostaining and C-peptide secretion. Cell transplantation experiments were performed with STZ-induced diabetic NOD/SCID mice. Blood human C-peptide and glucagon levels were measured serially in mice, and grafts were analyzed histologically.
resultsWe obtained spherical pancreatic beta-like cells from human iPS cells and detected verifiable amounts of C-peptide secretion
conclusionsThese results suggest that differentiated cells derived from human iPS cells grown in suspension culture mature and function like pancreatic islets
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.