ArticleMethodsX2018
Streamlining standard bacteriophage methods for higher throughput.
Article in MethodsX, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Complex temporal dynamics of phage-bacteria populations in an animal-associated marine system.Nature communications · 2026Article
- Phage infection fronts trigger early sporulation and viral entrapment in bacterial populations.The ISME journal · 2026Article
- A satellite in the phage universe: isolation and characterization of six novelFEMS microbes · 2026Article
- The ecology and evolution of microbial immune systems: a look on the wild vibrio side.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Abiotic environmental conditions determine phage resistance outcomes in a salt-marsh bacterium.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage-host interactions.PLoS biology · 2025Article
- Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches.The Journal of clinical investigation · 2025Review
- Adaptive genomic plasticity in large-genome, broad-host-range vibrio phages.The ISME journal · 2025Article
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- Article
- Expanding the Phage Galaxy: Isolation and Characterization of Five NovelPHAGE (New Rochelle, N.Y.) · 2024Article
- Seed coating with phages for sustainable plant biocontrol of plant pathogens and influence of the seed coat mucilage.Microbial biotechnology · 2024Article
- Germicidal efficacy of continuous and pulsed ultraviolet-C radiation on pathogen models and SARS-CoV-2.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Phage-inducible chromosomal minimalist islands (PICMIs), a novel family of small marine satellites of virulent phages.Nature communications · 2024Article
- Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication.Nature communications · 2024Article
- Isolation of Enterococcus Bacteriophages from Municipal Wastewater Samples Using an Enrichment Step.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Low-volume enrichment method supports high throughput bacteriophage screening and isolation from wastewater.PloS one · 2024Article
- Coevolution between marine Aeromonas and phages reveals temporal trade-off patterns of phage resistance and host population fitness.The ISME journal · 2023Article
- Bacteria-phage coevolution with a seed bank.The ISME journal · 2023Article
- Genetic determinants of host tropism in Klebsiella phages.Cell reports · 2023Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A universal tool in the culture-based study of bacterial viruses (bacteriophages, or phages) is the agar overlay, which is used in the isolation of new viruses, and in their quantification and purification. Here, simple optimizations that increase efficiency and throughput in agar overlay based isolation and cultivation of virus-host systems are presented. The agar overlay is streamlined to minimize steps and materials. Serial purification of viruses from viral colonies (plaques) is optimized to eliminate steps by combining purification by serial re-streaking with the optimized agar overlay approach. Finally, recommendations are made for efficient archival and storage of virus plaques. In sum, this work presents: •Tube-free Agar Overlays: rapid plaque assays with fewer steps and materials•Molten Streaking for Singles: rapid tube-free serial purification of viruses•Archiving Plaques: saving virus purification for later.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.