Evidence map›Paper›PMID 30621594›Full record

ArticleBMC microbiology2019

Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay.

Tao Wu, Haizhen Wu, Kangchen Zhao, Chaoyou Hu, Yiyue Ge, Xiaojuan Zhu, Xingchen Zhang, Minghao Zhou, Fengcai Zhu, Lunbiao Cui

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Preliminary Evaluation of Rapid Visual Identification ofFrontiers in cellular and infection microbiology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Tao WuInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.ORCID 0000-0001-9911-9009
Haizhen WuKunshan Municipal Center for Disease Control and Prevention, Kunshan, 215300, China.
Kangchen ZhaoInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Chaoyou HuKunshan Municipal Center for Disease Control and Prevention, Kunshan, 215300, China.
Yiyue GeInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Xiaojuan ZhuInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Xingchen ZhangKunshan Municipal Center for Disease Control and Prevention, Kunshan, 215300, China.
Minghao ZhouInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Fengcai ZhuInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Lunbiao CuiInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China. lbcui@jscdc.cn.
Jiangsu Provincial Center for Disease Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAs an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device).

resultsThis assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (< 15 min per detection). Furthermore, the real-time RPA assay has excellent correlation with real-time PCR assay for detection of HAdVs species B presented in clinical samples.

conclusionsThus, the RPA assay developed in this study provides an effective and portable approach for the rapid detection of HAdVs species B.

Indexed as

DNA-Directed DNA PolymeraseHumansLimit of DetectionMastadenovirusMolecular TypingNucleic Acid Amplification TechniquesPolymerase Chain ReactionRecombinasesReproducibility of ResultsVirologyDNA-Directed DNA PolymeraseRecombinasesHuman mastadenovirusesLateral-flow deviceReal-timeRecombinase polymerase amplification

Identifiers

PMID30621594
PMCPMC6325725
OpenAlexW2947398492

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.