ArticlePLoS neglected tropical diseases2019
Matching the genetics of released and local Aedes aegypti populations is critical to assure Wolbachia invasion.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers, 1 of them a synthesis that pooled it.
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Who cites it
101 citing papers in PubMed, 1 synthesis or guideline pooled it.
- What happens when we modify mosquitoes for disease prevention? A systematic review.Emerging microbes & infections · 2020Pooled it
- Trial
- Native Wolbachia infection dynamics across Aedes albopictus (Diptera: Culicidae) populations in Hawai'i.Parasites & vectors · 2026Article
- Establishment and characterization of a Wolbachia (wAlbB)-infected Aedes aegypti line (Tw-Kao) for dengue control.PLoS neglected tropical diseases · 2026Article
- Wolbachia wMel and wAlbB strains differentially impact the vector competence of Aedes aegypti with a Brazilian genetic background for DENV-1 virus.Parasites & vectors · 2026Article
- The impact of large-scale release ofLancet regional health. Americas · 2026Article
- Wolbachia introgression in Rio de Janeiro remains at sub-optimal levels 30 months after its decline: challenges in the sustainability of wMel interventions for dengue control.Memorias do Instituto Oswaldo Cruz · 2026Article
- Entomological Surveillance and Vector Competence: A Crucial Front in Dengue Prevention.Current topics in microbiology and immunology · 2026Review
- Production and shipment of wolbachia-infected eggs allows the control of aedes albopictus through the incompatible insect technique on a remote island.Communications biology · 2025Article
- Midgut microbiota diversity and interactions shift when field Aedes aegypti mosquitoes carry the wMel strain of Wolbachia pipientis.Communications biology · 2025Article
- Heterogeneity in inhibition of genetically diverse dengue virus strains bybioRxiv : the preprint server for biology · 2025Article
- Long-Term Durability and Public Health Impact of City-WideTropical medicine and infectious disease · 2025Article
- The long-term persistence of the wMel strain in Rio de Janeiro is threatened by poor integrated vector management and bacterium fitness cost on Aedes aegypti.PLoS neglected tropical diseases · 2025Article
- Review
- Changes in the frequency of facultative endosymbionts in insect populations: overview and applications.Entomologia generalis · 2025Article
- Does use of domestic insecticides undermine public health control strategies?Lancet regional health. Americas · 2025Review
- Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB.Scientific reports · 2025Article
- ExploitingPathogens (Basel, Switzerland) · 2025Review
- Development of the Incompatible Insect Technique targetingApplied and environmental microbiology · 2025Article
- Article
41 more citing papers are in PubMed but not listed here.
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17 authors.
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Abstract
backgroundTraditional vector control approaches such as source reduction and insecticide spraying have limited effect on reducing Aedes aegypti population. The endosymbiont Wolbachia is pointed as a promising tool to mitigate arbovirus transmission and has been deployed worldwide. Models predict a rapid increase on the frequency of Wolbachia-positive Ae. aegypti mosquitoes in local settings, supported by cytoplasmic incompatibility (CI) and high maternal transmission rate associated with the wMelBr strain. METHODOLOGY/PRINCIPLE
findingsWolbachia wMelBr strain was released for 20 consecutive weeks after receiving >87% approval of householders of the isolated community of Tubiacanga, Rio de Janeiro. wMelBr frequency plateued~40% during weeks 7-19, peaked 65% but dropped as releases stopped. A high (97.56%) maternal transmission was observed. Doubling releases and deploying mosquitoes with large wing length and low laboratory mortality produced no detectable effects on invasion trend. By investigating the lab colony maintenance procedures backwardly, pyrethroid resistant genotypes in wMelBr decreased from 68% to 3.5% after 17 generations. Therefore, we initially released susceptible mosquitoes in a local population highly resistant to pyrethroids which, associated with the over use of insecticides by householders, ended jeopardizing Wolbachia invasion. A new strain (wMelRio) was produced after backcrossing wMelBr females with males from field to introduce mostly pyrethroid resistance alleles. The new strain increased mosquito survival but produced relevant negative effects on Ae. aegypti fecundity traits, reducing egg clutche size and egg hatch. Despite the cost on fitness, wMelRio successful established where wMelBr failed, revealing that matching the local population genetics, especially insecticide resistance background, is critical to achieve invasion. CONCLUSIONS/SIGNIFICANCE: Local householders support was constantly high, reaching 90% backing on the second release (wMelRio strain). Notwithstanding the drought summer, the harsh temperature recorded (daily average above 30°C) did not seem to affect the expression of maternal transmission of wMel on a Brazilian background. Wolbachia deployment should match the insecticide resistance profile of the wild population to achieve invasion. Considering pyrethroid-resistance is a widely distributed phenotype in natural Ae. aegypti populations, future Wolbachia deployments must pay special attention in maintaining insecticide resistance in lab colonies for releases.
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