ArticleThe Histochemical journal1988
Lectin binding to rat spermatogenic cells: effects of different fixation methods and proteolytic enzyme treatment.
Article in The Histochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Lectins identify distinct populations of coelomocytes in Strongylocentrotus purpuratus.PloS one · 2017Article
- Immunohistochemical study of nuclear changes associated with male germ cell death and spermiogenesis.Journal of molecular histology · 2009Article
- Characterization of corpora amylacea glycoconjugates in normal and hyperplastic glands of human prostate.Journal of molecular histology · 2005Article
- Distribution of lectin binding in the testes of the musk shrew, Suncus murinus.Journal of anatomy · 1995Article
- Histochemical study of glycoconjugates in the epididymis of the hamster (Mesocricetus auratus).The Histochemical journal · 1995Article
- Lectin-binding carbohydrates in sexual differentiation of rat male and female gonads.Histochemistry · 1992Article
- Histochemical analysis of rat testicular glycoconjugates. 1. Subsets of N-linked saccharides in seminiferous tubules.The Histochemical journal · 1992Article
- Distribution of sugar residues in the bovine testis during postnatal ontogenesis demonstrated with lectin-horseradish peroxidase conjugates.Histochemistry · 1992Article
- Primary cultures of normal rat kidney proximal tubule epithelial cells for studies of renal cell injury.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1991Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The binding of a panel of eight different fluorescein-conjugated lectins to rat spermatogenic cells was investigated. Particular attention was paid to the effects of different fixation methods and proteolytic enzyme digestion on the staining pattern. Concanavalin A (Con A), wheatgerm agglutinin (WGA), succinylated WGA (s-WGA) and agglutinin from gorse (UEA I) stained the cytoplasm of most germ cells as well as the spermatid acrosome. In contrast, peanut agglutinin (PNA), castor bean agglutinin (RCAI) and soy bean agglutinin (SBA) mainly stained the acrosome. The staining pattern varied depending on the fixation method used. PNA was particularly sensitive to formalin fixation, while SBA, DBA and UEA I showed decreased binding and Con A, WGA, s-WGA and RCA I were insensitive to this type of fixation. Pepsin treatment of the sections before lectin staining caused marked changes in the staining pattern; staining with PNA in formalin-fixed tissue sections was particularly improved but there was also enhanced staining with SBA and horse gram agglutinin (DBA). On the other hand, in Bouin- and particularly in acetone-fixed tissue sections, pepsin treatment decreased the staining with several of the lectins, for example WGA and UEA I.
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