ArticleFrontiers in immunology2018
Perspective on the Development and Validation of Ab Reagents to Fish Immune Proteins for the Correct Assessment of Immune Function.
Article in Frontiers in immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Article
- Monoclonal antibody generated against Nile tilapia (Fish and shellfish immunology reports · 2023Article
- A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M.Fish and shellfish immunology reports · 2023Article
- Article
- RNA-Seq of Single Fish Cells - Seeking Out the Leukocytes Mediating Immunity in Teleost Fishes.Frontiers in immunology · 2022Review
- Isolation, Identification, and Investigation of Pathogenic Bacteria From Common Carp (Frontiers in immunology · 2022Article
- Mucosal immunoglobulins of teleost fish: A decade of advances.Developmental and comparative immunology · 2021Review
- Potential Applications of Fluorescence-Activated Cell Sorting (FACS) and Droplet-Based Microfluidics in Promoting the Discovery of Specific Antibodies for Characterizations of Fish Immune Cells.Frontiers in immunology · 2021Review
- Fish and shellfish immunology reports: A new open access journal.Fish and shellfish immunology reports · 2020Article
- Properties of Carotenoids in Fish Fitness: A Review.Marine drugs · 2020Review
- Review
- Antibodies, Nanobodies, or Aptamers-Which Is Best for Deciphering the Proteomes of Non-Model Species?International journal of molecular sciences · 2020Review
- The Veterinary Immunological Toolbox: Past, Present, and Future.Frontiers in immunology · 2020Review
- Evidence of Trained Immunity in a Fish: Conserved Features in Carp Macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Dual RNA-Seq UnveilsFrontiers in immunology · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Understanding of immune function in humans and model organisms, such as mice, has advanced in the last few decades because of technological breakthroughs and availability of reagents. While novel genomic technologies have helped to increase knowledge of many aspects of immunology, most developments in immunology have occurred because of the availability of antibodies to identify and sort different cell types, as well as to identify and quantify the protein products of cells. Unfortunately, many studies performed in fish make use of poorly characterized antibody reagents that may affect the conclusions of those studies. In light of this, we would like to offer some insight and discussion points based on our research experience on the strategies and techniques that are required for proper validation of antibody reagents to fish immune molecules. Our main goal is to encourage a much needed discussion in our field to foster the use of correctly validated reagents that enable the study of fish immune function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.