ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2019
Expression of fluconazole resistance-associated genes in biofilm from 23 clinical isolates of Candida albicans.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Natural-Compound Adjuvants Dismantle Candida Biofilms: Mechanisms, Design Rules, and Biofilm-Aware Pharmacology.Current microbiology · 2026Review
- Interspecies Interactions of Single- and Mixed-Species Biofilms ofBiomedicines · 2025Article
- Study on the impact of biofilm formation byFrontiers in cellular and infection microbiology · 2025Article
- Possible Molecular Targeting of Biofilm-Associated Genes by Nano-Ag in Candida albicans.Applied biochemistry and biotechnology · 2024Article
- Photoinactivation of microorganisms using bacteriochlorins as photosensitizers.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Anti-Biofilm Activity of Phenyllactic Acid against Clinical Isolates of Fluconazole-ResistantJournal of fungi (Basel, Switzerland) · 2023Article
- Emergence of Antifungal Resistant Subclades in the Global Predominant Phylogenetic Population of Candida albicans.Microbiology spectrum · 2023Article
- Distribution and antifungal susceptibility pattern ofVirulence · 2022Review
- Effects ofAntibiotics (Basel, Switzerland) · 2022Article
- Investigation of fluconazole susceptibility to Candida albicans by MALDI-TOF MS and real-time PCR for CDR1, CDR2, MDR1 and ERG11.BMC microbiology · 2022Article
- Repurposing pantoprazole and haloperidol as efflux pump inhibitors in azole resistant clinicalSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2022Article
- Article
- Glass ionomer cement modified by a imidazolium salt: adding antifungal properties to a biomaterial.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Fungal Biofilms as a Valuable Target for the Discovery of Natural Products That Cope with the Resistance of Medically Important Fungi-Latest Findings.Antibiotics (Basel, Switzerland) · 2021Review
- Inhibition of Phospholipase by Orlistat as an Alternate Therapy to Combat Opportunistic Mycosis Caused by C. albicans.Current microbiology · 2021Article
- Cotreatment with Aspirin and Azole Drugs Increases Sensitivity ofInfection and drug resistance · 2021Article
- Application of Probiotic Yeasts onJournal of fungi (Basel, Switzerland) · 2020Review
- In vitro Antibiotic and Modulatory Activity ofAntibiotics (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to establish the influence of biofilm from clinical isolates of Candida albicans on fluconazole resistance, focusing on efflux pumps and azole-targeted enzymes. Twenty-three C. albicans clinical isolates were collected from two hospitals in Shanghai, China. Antifungal susceptibility tests were performed on biofilm and planktonic cells. A crystal violet assay was used to monitor biofilm growth. Real-time RT-PCR was performed to quantify the expression of the transporter-related genes MDR1, CDR1, and CDR2 as well as ERG11, a gene encoding an enzyme targeted by antifungal drugs. Fluconazole resistance was shown to increase in biofilm in a time-dependent manner. No significant differences were observed between different strains of C. albicans. Genes encoding efflux pumps were overexpressed in early stages of biofilm formation and could also be induced by fluconazole. While ERG11 was not upregulated in biofilm, it was overexpressed upon the addition of fluconazole to biofilm and planktonic cells. Gene expression also appeared to be related to the original genotype of the strain. The upregulation of genes encoding efflux pumps demonstrates their role in the development of fluconazole resistance during the early stages of C. albicans biofilm formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.