ArticlePlant molecular biology2019
WRKY12 represses GSH1 expression to negatively regulate cadmium tolerance in Arabidopsis.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 129 citations in OpenAlex.
- Transcription Factor TCP9 EnhancesPlants (Basel, Switzerland) · 2026Article
- Advances in Molecular Mechanisms Underlying Cadmium Accumulation and Detoxification in Durum Wheat (International journal of molecular sciences · 2026Review
- WRKY transcription factors govern selenium metabolism in Broussonetia papyrifera: genome-wide identification, expression profiling, and regulatory network analysis.BMC plant biology · 2026Article
- RsWRKY15-RsPDR12 module regulates Cd uptake and accumulation by promoting Cd efflux in radish (Raphanus sativus L.).Molecular horticulture · 2026Article
- Domestication-Selected Promoter Insertion in WRKY17 Increases Cadmium Sensitivity in Apple.Plant biotechnology journal · 2026Article
- The Maize WRKY Transcription Factor ZmWRKY4 Confers Lead Tolerance by RegulatingPlants (Basel, Switzerland) · 2026Article
- Plant Responses to Heavy Metal Stresses: Mechanisms, Defense Strategies, and Nanoparticle-Assisted Remediation.Plants (Basel, Switzerland) · 2025Review
- Genome-wide characterization of NRAMP metal transporters reveals functional diversity for heavy metal tolerance in Onobrychis viciifolia.BMC genomics · 2025Article
- GSH1 plays a crucial role in melatonin-mediated cadmium tolerance in Arabidopsis.Plant cell reports · 2025Article
- Selenium Alleviates Cadmium Toxicity by Regulating Carbon Metabolism, AsA-GSH Cycle, and Cadmium Transport inPlants (Basel, Switzerland) · 2025Article
- RsWRKY75 promotes ROS scavenging and cadmium efflux via activating the transcription of RsAPX1 and RsPDR8 in radish (Raphanus sativus L.).Plant cell reports · 2025Article
- Genome wide identification of MATE and ALMT gene family in lentil (Lens culinaris Medikus) and expression profiling under Al stress condition.BMC plant biology · 2025Article
- Genome-wide identiffcation of the WRKY gene family in poplar and the positive role of PsnWRKY95 in response to cadmium stress.PloS one · 2025Article
- PcWRKY1 Represses Transcription of Yellow Stripe-Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Lead toxicity in plants: mechanistic insights into toxicity, physiological responses of plants and mitigation strategies.Plant signaling & behavior · 2024Review
- Evolution of Duplicated Glutathione Metabolic Pathway inEcology and evolution · 2024Article
- WRKY Transcription Factors in Response to Metal Stress in Plants: A Review.International journal of molecular sciences · 2024Review
- An Overview of the Mechanisms through Which Plants Regulate ROS Homeostasis under Cadmium Stress.Antioxidants (Basel, Switzerland) · 2024Review
- Identification, characterization, and expression analysis of WRKY transcription factors in Cardamine violifolia reveal the key genes involved in regulating selenium accumulation.BMC plant biology · 2024Article
- Metal transport proteins and transcription factor networks in plant responses to cadmium stress.Plant cell reports · 2024Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
key messageThe WRKY transcription factor WRKY12 negatively regulates Cd tolerance in Arabidopsis via the glutathione-dependent phytochelatin synthesis pathway by directly targeting GSH1 and indirectly repressing phytochelatin synthesis-related gene expression. Cadmium (Cd) is a widespread pollutant toxic to plants. The glutathione (GSH)-dependent phytochelatin (PC) synthesis pathway plays key roles in Cd detoxification. However, its regulatory mechanism remains largely unknown. Here, we showed a previously unknown function of the WRKY transcription factor WRKY12 in the regulation of Cd tolerance by repressing the expression of PC synthesis-related genes. The expression of WRKY12 was inhibited by Cd stress. Enhanced Cd tolerance was observed in the WRKY12 loss-of-function mutants, whereas increased Cd sensitivity was found in the WRKY12-overexpressing plants. Overexpression and loss-of-function of WRKY12 were associated respectively with increased and decreased Cd accumulation by repressing or releasing the expression of the genes involved in the PC synthesis pathway. Transient expression assay showed that WRKY12 repressed the expression of GSH1, GSH2, PCS1, and PCS2. Further analysis indicated that WRKY12 could directly bind to the W-box of the promoter in GSH1 but not in GSH2, PCS1, and PCS2 in vivo. Together, our results suggest that WRKY12 directly targets GSH1 and indirectly represses PC synthesis-related gene expression to negatively regulate Cd accumulation and tolerance in Arabidopsis.
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