ArticleAnalytical and bioanalytical chemistry2019
Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Reproducibility crisis in isothermal amplification: lessons from benchmarking LAMP assays.Microbiology spectrum · 2026Article
- Trends of nucleic acid - based point-of-care diagnostics for infectious diseases.Journal of biological engineering · 2026Review
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Bacteria as living biosensors for DNA.Nature reviews bioengineering · 2026Article
- Rapid, sensitive, and highly specific diagnosis of respiratory syncytial virus using recombinase polymerase amplification-based biosensor and fluorescence detection.Frontiers in cellular and infection microbiology · 2026Article
- Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis.PLoS neglected tropical diseases · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.Scientific reports · 2025Article
- Isothermal Amplification Techniques: An Emerging Tool for On-Site Detection of Phytopathogens in Field Conditions.Methods in molecular biology (Clifton, N.J.) · 2025Review
- A novel ionic liquid-based approach for DNA and RNA extraction simplifies sample preparation for bacterial diagnostics.Analytical and bioanalytical chemistry · 2024Article
- Real-Time Visualization of HIV-1 RNA Detection Using Loop-Mediated Isothermal Amplification-Enabled Particle Diffusometry.ACS sensors · 2024Article
- Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health.Molecular biotechnology · 2024Review
- Ensuring food safety: Microfluidic-based approaches for the detection of food contaminants.Analytical science advances · 2024Review
- Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection ofFoods (Basel, Switzerland) · 2023Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Rapid Detection of Porcine DNA in Meatball Using Recombinase Polymerase Amplification Couple with Lateral Flow Immunoassay for Halal Authentication.Molecules (Basel, Switzerland) · 2022Article
- Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis.Analytical and bioanalytical chemistry · 2022Article
- Diagnostics of COVID-19 Based on CRISPR-Cas Coupled to Isothermal Amplification: A Comparative Analysis and Update.Diagnostics (Basel, Switzerland) · 2022Review
- Isothermal Amplification of Nucleic Acids Coupled with Nanotechnology and Microfluidic Platforms for Detecting Antimicrobial Drug Resistance and Beyond.Advanced pharmaceutical bulletin · 2022Review
- Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples.Scientific reports · 2021Observational
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular diagnostic tools in the field of food and water quality analysis are becoming increasingly widespread. Usually, based on DNA amplification techniques such as polymerase chain reaction (PCR), these methods are highly sensitive and versatile but require well-equipped laboratories and trained personnel. To reduce analysis time and avoid expensive equipment, isothermal DNA amplification methods for detecting various target organisms have been developed. However, to make molecular diagnostics suitable for low-resource settings and in-field applications, it is crucial to continuously adapt the working steps associated with DNA amplification, namely sample preparation, DNA extraction, and visualization of the results. Many novel approaches have been evaluated in recent years to tackle these challenges, e.g., the use of ionic liquids for the rapid isolation of nucleic acids from organisms relevant for food and water analysis or the integration of entire analytical workflows on microfluidic chips. In any event, the future of applications in the field of isothermal amplification will probably lie in ready-to-use cartridges combined with affordable handheld devices for on-site analysis. This trend article aims to make prospective users more familiar with this technology and its potential for moving molecular diagnostics from the laboratory to the field. Graphical abstract ᅟ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.