Evidence map›Paper›PMID 30617347›Full record

ArticleNature microbiology2019

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.

Jason M Peters, Byoung-Mo Koo, Ramiro Patino, Gary E Heussler, Cameron C Hearne, Jiuxin Qu, Yuki F Inclan, John S Hawkins, Candy H S Lu, Melanie R Silvis and 8 more

Open access · bronzeAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.

0numbers the graph read from it
0cells of the map it votes in
145citing papers in PubMed
7.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

145 citing papers in PubMed, 275 citations in OpenAlex.

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  15. Functional Genomics: Principles and Methods.Advances in experimental medicine and biology · 2026
    Review
  16. Review
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85 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 2 countries.

Jason M PetersDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. jason.peters@wisc.edu.ORCID http://orcid.org/0000-0003-2722-8382
Byoung-Mo KooDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Ramiro PatinoDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Gary E HeusslerDivision of Biological Sciences, University of California, San Diego, San Diego, CA, USA.
Cameron C HearneDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Jiuxin QuDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Yuki F InclanDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
John S HawkinsDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Candy H S LuDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Melanie R SilvisDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
M Michael HardenDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Hendrik OsadnikDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Joseph E PetersDepartment of Microbiology, Cornell University, Ithaca, NY, USA.ORCID http://orcid.org/0000-0001-8309-3297
Joanne N EngelDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Rachel J DuttonDivision of Biological Sciences, University of California, San Diego, San Diego, CA, USA.
Alan D GrossmanDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-8235-7227
Carol A GrossDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. cgrossucsf@gmail.com.ORCID http://orcid.org/0000-0002-5595-9732
Oren S RosenbergDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. oren.rosenberg@ucsf.edu.ORCID http://orcid.org/0000-0002-5736-4388
University of California, San Francisco · USMassachusetts Institute of Technology · USUniversity of San Diego · USUniversity of Wisconsin–Madison · USQB3 · US

Funding

PRE-DOCTORAL TRAINING IN THE BIOLOGICAL SCIENCEST32GM007287 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BELL, STEPHEN P., KEATING, AMY E · 1985 to 2019
$40.8M
Cellular homeostasis pathways in bacteriaR35GM118061 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, CAROL ANNE · 2016 to 2025
$9.0M
Microbial Pathogenesis and Host DefenseT32AI060537 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Joanne N. Engel · 2004 to 2026
$6.5M
Novel Imaging Tracers for Rapid and Noninvasive Assessment of Bacterial InfectionR01EB020539 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI JAIN, SANJAY · 2014 to 2018
$3.6M
Molecular mechanisms that shape microbial communitiesDP2AT010401 · NCCIH · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DUTTON, RACHEL J. · 2018 to 2018
$2.4M
Clinical translation of D-amino acid derived PET tracers for imaging spinalinfectionR01EB024014 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI OHLIGER, MICHAEL, WILSON, DAVID M · 2017 to 2020
$2.2M
Protein secretion in bacterial pathogensR01AI128214 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, CAROL ANNE · 2017 to 2021
$2.0M
Gene Function and Pathway Analysis Using Systems Level Approaches in ProkaryotesR01GM102790 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, CAROL ANNE · 2012 to 2015
$1.8M
Systematic Phenotypic Analysis of the Gram-positive EnvelopeF32GM108222 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PETERS, JASON M. · 2013 to 2016
$161k
NCCIH NIH HHS DP2 AT010401NIAID NIH HHS R01 AI128214NIAID NIH HHS T32 AI060537NIGMS NIH HHS F32 GM108222NIGMS NIH HHS R01 GM102790NIGMS NIH HHS R35 GM118061NIGMS NIH HHS T32 GM007287
6 · The paper itself

Abstract

The vast majority of bacteria, including human pathogens and microbiome species, lack genetic tools needed to systematically associate genes with phenotypes. This is the major impediment to understanding the fundamental contributions of genes and gene networks to bacterial physiology and human health. Clustered regularly interspaced short palindromic repeats interference (CRISPRi), a versatile method of blocking gene expression using a catalytically inactive Cas9 protein (dCas9) and programmable single guide RNAs, has emerged as a powerful genetic tool to dissect the functions of essential and non-essential genes in species ranging from bacteria to humans

Indexed as

CRISPR-Cas SystemsGenetic TechniquesAnti-Bacterial AgentsBacteriaBacterial ProteinsBacteriological TechniquesConjugation, GeneticDrug Resistance, MicrobialGene LibraryGene Regulatory NetworksGenes, EssentialGene TargetingGenome, BacterialAnti-Bacterial AgentsBacterial Proteins

Identifiers

PMID30617347
PMCPMC6424567
OpenAlexW2906110636

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.