ArticleBMC research notes2019
Development of a new method for collecting hemolymph and measuring phenoloxidase activity in Tribolium castaneum.
Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular and Functional Characterization of Black Soldier Fly Derived Peptide Against Food-Relevant Bacteria.Foods (Basel, Switzerland) · 2026Article
- Alternative High-Quality Hemolymph Extraction from AdultInsects · 2026Article
- A Simple Method for Estimating the Spatiotemporal Distribution of Phenoloxidase Proteins in Insect Tissues.Bio-protocol · 2026Article
- Exploring the role of microbiota in mediating sexually dimorphic infection outcomes in mealworm beetles.Biology letters · 2025Article
- Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome.Biological research · 2024Article
- TGF-β Type II Receptor Punt Suppresses Antimicrobial Peptide Expression and Influences Development inInsects · 2023Article
- Article
- Immune Responses of the Black Soldier FlyLife (Basel, Switzerland) · 2023Article
- An Efficient and Simple Method for Collecting Haemolymph of Cerambycidae (Insecta: Coleoptera) Adults.Insects · 2022Article
- Efficient Oral Priming ofVaccines · 2022Article
- Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1.Insect biochemistry and molecular biology · 2022Article
- Superoxide dismutase 6 is required during metamorphosis for the development of properly movable legs in Tribolium castaneum.Scientific reports · 2022Article
- Article
- A unique Malpighian tubule architecture inProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
objectiveHemolymph plays many important roles in the physiology of an insect throughout its lifetime; however, for small-bodied insects, studies are lacking because of the difficulties encountered while collecting hemolymph. The objective of our study was to develop a method to collect hemolymph plasma from various stages of Tribolium castaneum and to evaluate phenoloxidase activity in the plasma samples. We first designed a procedure for easily and quickly collecting clear hemolymph plasma from T. castaneum.
resultsBy using this method, we collected approximately 5 µl plasma from 30 individuals at the larval, pupal or adult stages. And then, we studied the expression of phenoloxidase by performing western blot analysis of the plasma samples and found that phenoloxidase is present in hemolymph in each developmental stage. We also measured phenoloxidase activity in control plasma and plasma treated with Gram-positive bacteria, Micrococcus luteus. Phenoloxidase activity was greater in some of the M. luteus-treated plasma samples compared with control samples. Thus, we developed a method to collect hemolymph plasma that is suitable for studies of phenoloxidase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.