Evidence map›Paper›PMID 30616506›Full record

ArticleBMC immunology2019

Human-like NSG mouse glycoproteins sialylation pattern changes the phenotype of human lymphocytes and sensitivity to HIV-1 infection.

Raghubendra Singh Dagur, Amanda Branch-Woods, Saumi Mathews, Poonam S Joshi, Rolen M Quadros, Donald W Harms, Yan Cheng, Shana M Miles, Samuel J Pirruccello, Channabasavaiah B Gurumurthy and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Raghubendra Singh DagurDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Amanda Branch-WoodsDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Saumi MathewsDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Poonam S JoshiMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, Omaha, NE, USA.
Rolen M QuadrosMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, Omaha, NE, USA.
Donald W HarmsMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, Omaha, NE, USA.
Yan ChengDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Shana M MilesBellevue Medical Center, Bellevue, NE, USA.
Samuel J PirruccelloDepartment of Pathology and Microbiology, Omaha, NE, USA.
Channabasavaiah B GurumurthyMouse Genome Engineering Core Facility, Vice Chancellor for Research Office, Omaha, NE, USA.
Santhi GorantlaDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Larisa Y PoluektovaDepartment of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198, USA. lpoluekt@unmc.edu.ORCID 0000-0001-7339-8732
University of Nebraska Medical Center · USOffice of the Director · USNebraska Medical Center · USBellevue University · USUniversity of Nebraska at Omaha · US

Funding

Center for Humanized MiceR24OD018546 · OD · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI GORANTLA, SANTHI, POLUEKTOVA, LARISA Y · 2014 to 2017
$3.2M
NIH HHS R24 OD018546
6 · The paper itself

Abstract

backgroundThe use of immunodeficient mice transplanted with human hematopoietic stem cells is an accepted approach to study human-specific infectious diseases such as HIV-1 and to investigate multiple aspects of human immune system development. However, mouse and human are different in sialylation patterns of proteins due to evolutionary mutations of the CMP-N-acetylneuraminic acid hydroxylase (CMAH) gene that prevent formation of N-glycolylneuraminic acid from N-acetylneuraminic acid. How changes in the mouse glycoproteins' chemistry affect phenotype and function of transplanted human hematopoietic stem cells and mature human immune cells in the course of HIV-1 infection are not known.

resultsWe mutated mouse CMAH in the NOD/scid-IL2Rγ

conclusionNSG-cmah

Indexed as

HIV-1AnimalsCRISPR-Cas SystemsDisease Models, AnimalGenetic LociGlycoproteinsHematopoietic Stem CellsHematopoietic Stem Cell TransplantationHIV InfectionsImmune SystemImmunophenotypingLeukocytes, MononuclearLymphocytesMiceMice, KnockoutPhenotypeGlycoproteinsCMP-N-acetylneuraminic acid hydroxylaseHematopoietic stem cellsHIV-1NOD/scid-IL2Rγc −/− mice

Identifiers

PMID30616506
PMCPMC6322283
OpenAlexW2889034841

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.