ArticleVirology2019
A reverse-transcription/RNase H based protocol for depletion of mosquito ribosomal RNA facilitates viral intrahost evolution analysis, transcriptomics and pathogen discovery.
Article in Virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Evidence of dengue transmission and a diverse Aedes mosquito virome on the Congo's Angola border.Nature communications · 2026Article
- Genomic characterization of a severe West Nile Virus transmission season using a single reaction amplicon sequencing approach.PLoS neglected tropical diseases · 2026Article
- Phylogenetic insights into the transmission dynamics of arthropod-borne viruses.Nature reviews. Genetics · 2026Review
- Virome sequencing and analysis of Aedes aegypti and Aedes albopictus from ecologically different sites in the Philippines.Parasites & vectors · 2025Article
- Evidence of dengue virus transmission and a diversebioRxiv : the preprint server for biology · 2025Article
- Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors.Nature communications · 2025Article
- Influenza virus antagonizes self sensing by RIG-I to enhance viral replication.bioRxiv : the preprint server for biology · 2025Article
- Ribosomal RNA Depletion for Poly(A)-Tail-Independent Quantification of Genome Activation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Y-switch: a spring-loaded synthetic gene switch for robust DNA/RNA signal amplification and detection.Nucleic acids research · 2024Article
- The 28S rRNA RT-qPCR assay for host depletion evaluation to enhance avian virus detection in Illumina and Nanopore sequencing.Frontiers in microbiology · 2024Article
- Evaluation of Vector-Enabled Xenosurveillance in Rural Guatemala.The American journal of tropical medicine and hygiene · 2023Article
- Article
- Non-target RNA depletion strategy to improve sensitivity of next-generation sequencing for the detection of RNA viruses in poultry.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Uncovering the Worldwide Diversity and Evolution of the Virome of the MosquitoesMicroorganisms · 2021Article
- Optimized design of antisense oligomers for targeted rRNA depletion.Nucleic acids research · 2021Article
- High Throughput Sequencing for the Detection and Characterization of RNA Viruses.Frontiers in microbiology · 2021Review
- RNAflow: An Effective and Simple RNA-Seq Differential Gene Expression Pipeline Using Nextflow.Genes · 2020Article
- Ribo-Pop: simple, cost-effective, and widely applicable ribosomal RNA depletion.RNA (New York, N.Y.) · 2020Article
- Narnaviruses: novel players in fungal-bacterial symbioses.The ISME journal · 2020Article
- Virome Analysis of Aphid Populations That Infest the Barley Field: The Discovery of Two Novel Groups of Nege/Kita-Like Viruses and Other Novel RNA Viruses.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Identifying novel viruses or assessing viral variation by NGS requires high sequencing coverage. More than 90% of total RNA is ribosomal (rRNA), making variant calling, virus discovery or transcriptomic profiling difficult. Current methods to increase informative reads suffer from drawbacks, either they cannot be used for some viruses, are optimized for a single species, or introduce bias. We describe a two-part approach combining reverse-transcription to create RNA/DNA hybrids which are then degraded with RNaseH/DNase sequentially that works for three medically relevant mosquito genera; Aedes, Anopheles, and Culex. We demonstrate depletion of rRNA from different samples, including whole mosquitoes and midgut contents from FTA cards. We describe novel insect-specific virus genomes from field collected mosquitoes. The protocol requires only common laboratory reagents and small oligonucleotides specific to rRNA. This approach can be adapted for other organisms, aiding virus diversity analyses, virus discovery and transcriptomics in both laboratory and field samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.