ArticleBiomedical optics express2018
Miniature scanning light-sheet illumination implemented in a conventional microscope.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Nominally identical microplastic models differ greatly in their particle-cell interactions.Nature communications · 2024Article
- OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.Communications biology · 2023Article
- Volumetric light sheet imaging with adaptive optics correction.Biomedical optics express · 2023Article
- A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems.Scientific reports · 2022Article
- Smart imaging to empower brain-wide neuroscience at single-cell levels.Brain informatics · 2022Review
- Meta-lens light-sheet fluorescence microscopy forNanophotonics (Berlin, Germany) · 2022Article
- MEMS enabled miniaturized light-sheet microscopy with all optical control.Scientific reports · 2021Article
- Single-objective high-resolution confocal light sheet fluorescence microscopy for standard biological sample geometries.Biomedical optics express · 2021Article
- Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP.Biophysical journal · 2020Article
- Optical Tweezers Exploring Neuroscience.Frontiers in bioengineering and biotechnology · 2020Review
- VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.Optics express · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Living cells are highly dynamic systems responding to a large variety of biochemical and mechanical stimuli over minutes, which are well controlled by e.g. optical tweezers. However, live cell investigation through fluorescence microscopy is usually limited not only by the spatial and temporal imaging resolution but also by fluorophore bleaching. Therefore, we designed a miniature light-sheet illumination system that is implemented in a conventional inverted microscope equipped with optical tweezers and interferometric tracking to capture 3D images of living macrophages at reduced bleaching. The horizontal light-sheet is generated with a 0.12 mm small cantilevered mirror placed at 45° to the detection axis. The objective launched illumination beam is reflected by the micro-mirror and illuminates the sample perpendicular to the detection axis. Lateral and axial scanning of both Gaussian and Bessel beams, together with an electrically tunable lens for fast focusing, enables rapid 3D image capture without moving the sample or the objective lens. Using scanned Bessel beams and line-confocal detection, an average axial resolution of 0.8 µm together with a 10-15 fold improved image contrast is achieved.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.