ArticleAngewandte Chemie (International ed. in English)2019
Boron-Containing Probes for Non-optical High-Resolution Imaging of Biological Samples.
Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Boron-10 carriers and their applications in boron neutron capture therapy.Precision radiation oncology · 2026Review
- Vertex Engineering of the Dodecaborate-Based Broadband Membrane Carriers.Inorganic chemistry · 2025Article
- Mass spectrometry imaging of metals in tissues and cells: Methods and biological applications.Biochimica et biophysica acta. General subjects · 2024Review
- Proposal of recommended experimental protocols for in vitro and in vivo evaluation methods of boron agents for neutron capture therapy.Journal of radiation research · 2023Article
- A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry.International journal of molecular sciences · 2022Article
- Chemical Imaging and Analysis of Single Nerve Cells by Secondary Ion Mass Spectrometry Imaging and Cellular Electrochemistry.Frontiers in synaptic neuroscience · 2022Review
- Subcellular localization of biomolecules and drug distribution by high-definition ion beam imaging.Nature communications · 2021Article
- Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry.Nanomaterials (Basel, Switzerland) · 2021Article
- Single cell imaging reveals cisplatin regulating interactions between transcription (co)factors and DNA.Chemical science · 2021Article
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover.BMC molecular and cell biology · 2021Article
- Article
- Quantum Defects as a Toolbox for the Covalent Functionalization of Carbon Nanotubes with Peptides and Proteins.Angewandte Chemie (International ed. in English) · 2020Article
- Novel Secondary Ion Mass Spectrometry Methods for the Examination of Metabolic Effects at the Cellular and Subcellular Levels.Frontiers in behavioral neuroscience · 2020Article
- Generation and Application of Fluorescent Anti-Human β2-Microglobulin VHHs via Amino Modification.Molecules (Basel, Switzerland) · 2019Article
- Boron-Containing Probes for Non-optical High-Resolution Imaging of Biological Samples.Angewandte Chemie (International ed. in English) · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Boron has been employed in materials science as a marker for imaging specific structures by electron energy loss spectroscopy (EELS) or secondary ion mass spectrometry (SIMS). It has a strong potential in biological analyses as well; however, the specific coupling of a sufficient number of boron atoms to a biological structure has proven challenging. Herein, we synthesize tags containing closo-1,2-dicarbadodecaborane, coupled to soluble peptides, which were integrated in specific proteins by click chemistry in mammalian cells and were also coupled to nanobodies for use in immunocytochemistry experiments. The tags were fully functional in biological samples, as demonstrated by nanoSIMS imaging of cell cultures. The boron signal revealed the protein of interest, while other SIMS channels were used for imaging different positive ions, such as the cellular metal ions. This allows, for the first time, the simultaneous imaging of such ions with a protein of interest and will enable new biological applications in the SIMS field.
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