Evidence map›Paper›PMID 30613966›Full record

ArticleJournal of cellular physiology2019

KCNQ1 rescues TMC1 plasma membrane expression but not mechanosensitive channel activity.

William T Harkcom, Maria Papanikolaou, Vikram Kanda, Shawn M Crump, Geoffrey W Abbott

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. AProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

William T HarkcomPharmacology Department, Weill Medical College of Cornell University, New York, New York.
Maria PapanikolaouBioelectricity Laboratory, Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, California.
Vikram KandaPharmacology Department, Weill Medical College of Cornell University, New York, New York.
Shawn M CrumpBioelectricity Laboratory, Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, California.
Geoffrey W AbbottBioelectricity Laboratory, Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, California.ORCID 0000-0003-4552-496X
University of California, Irvine · USCornell University · US

Funding

Discovering the function of a putative ion channel family linked to inherited diseasesR21DC015982 · NIDCD · UNIVERSITY OF CALIFORNIA-IRVINE · PI ABBOTT, GEOFFREY W · 2017 to 2018
$425k
NIDCD NIH HHS R21 DC015982
6 · The paper itself

Abstract

Transmembrane channel-like protein isoform 1 (TMC1) is essential for the generation of mechano-electrical transducer currents in hair cells of the inner ear. TMC1 disruption causes hair cell degeneration and deafness in mice and humans. Although thought to be expressed at the cell surface in vivo, TMC1 remains in the endoplasmic reticulum when heterologously expressed in standard cell lines, precluding determination of its roles in mechanosensing and pore formation. Here, we report that the KCNQ1 Kv channel forms complexes with TMC1 and rescues its surface expression when coexpressed in Chinese Hamster Ovary cells. TMC1 rescue is specific for KCNQ1 within the KCNQ family, is prevented by a KCNQ1 trafficking-deficient mutation, and is influenced by KCNE β subunits and inhibition of KCNQ1 endocytosis. TMC1 lowers KCNQ1 and KCNQ1-KCNE1 K

Indexed as

Amino Acid MotifsAnimalsCell MembraneChlorocebus aethiopsCHO CellsCOS CellsCricetulusFemaleHair Cells, Auditory, InnerHumansKCNQ1 Potassium ChannelMechanotransduction, CellularMembrane PotentialsMembrane ProteinsMiceMutagenesis, Site-DirectedKCNE1 protein, humanKCNQ1 Potassium ChannelKCNQ1 protein, humanMembrane ProteinsPotassium Channels, Voltage-GatedRecombinant ProteinsTMC1 protein, humanKv7.1potassium channeltransmembrane channel-like protein isoform 1 (TMC1)

Identifiers

PMID30613966
PMCPMC6478532
OpenAlexW2910736690

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.